LOCUS       sequence-102983-         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  T7-transcription template for CAT control mRNA used for in vitro 
            translation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Rinaldi AJ, Lund PE, Blanco MR, Walter NG
  TITLE     The Shine-Dalgarno sequence of riboswitch-regulated single mRNAs 
            shows ligand-dependent accessibility bursts.
  JOURNAL   Nat Commun. 2016 Jan 19;7:8976. doi: 10.1038/ncomms9976.
  PUBMED    26781350
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-01-19"; volume: "7:8976. doi"; pages: " 
            10.1038/ncomms9976"
COMMENT     Sequence Label: pAMB_CAT-FspI
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..40)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(90..107)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(108..764)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     679..698
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     RBS             772..794
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(825..844)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(826..844)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctttgttagc agccggatct cagtggtggt ggtggtggtg ctcgagtgcg gccgcaagct
       61 tgtcgacgga gctcgaattc ggatccttaa tgatgatgat gatgatgcgc cccgccctgc
      121 cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa
      181 acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat
      241 ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa
      301 ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta
      361 gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac
      421 tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg
      481 aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc
      541 atacggaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa
      601 aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc
      661 tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat
      721 tgggatatat caacggtggt atatccagtg atttttttct ccatatgtat atctccttct
      781 taaagttaaa caaaattatt tctagaggga aaccgttgtg gtctccctat agtgagtcgt
      841 attaatttcg cgggatcgag atctgccagc cccgacaccc gccaacaccc gctgacgcgc
      901 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccgg
//
