LOCUS       sequence-102985-         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Non-fluorescent, truncated kinesin-1 fused to split GFP linker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Norris SR, Soppina V, Dizaji AS, Schimert KI, Sept D, Cai D, 
            Sivaramakrishnan S, Verhey KJ
  TITLE     A method for multiprotein assembly in cells reveals independent 
            action of kinesins in complex.
  JOURNAL   J Cell Biol. 2014 Nov 10;207(3):393-406. doi: 10.1083/jcb.201407086.
            Epub 2014 Nov 3.
  PUBMED    25365993
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201407086"; journalName: "J Cell Biol"; date: 
            "2014-11-10- 10"; volume: "207"; issue: "3"; pages: "393-406"
COMMENT     Sequence Label: KIF5C(1-560)-GFP11
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(118..165)
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
ORIGIN
        1 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
       61 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc tagatcaggt
      121 gatgccggcg gcgttcacgt actcgtgcag caccatgtgg tctctgccgc tgcctaccgg
      181 tcctccacct cctcctccac cacctcccgg taccagctcg cccagatcct tgagaagcag
      241 gttcaggatc tccgtggccc tcttcttctg gtggttgcta agctcctgta gctgactcag
      301 ctcccgctgg gtggttgtca gtgtcgtcgt tttctgggcc agctcatcgg tcagttgctc
      361 gttggcccgg gtcttgtcct ccacttcctg tgacttctgg tcgtaattga cagccagctc
      421 ctccagggcc tggaggacct ccttcacctc gtctttagcc gcctcgtttt caatctggag
      481 gcgggtcagc tcctcctgga ttttctcgta gtcccttctg gtggaagcta gaagctcgtc
      541 ctgatccaac atctgttgct tcagcttttc agccagctgg ctctgctggt taatttcatc
      601 gtccttgtca tccagctgtc ggtacagact ggtgatttcc tcgtcatact tctctttctc
      661 agcagagatg ccgtcaacca caggtgtgat gttgtctatg atgggtgtgt tgtcgcaggg
      721 ctccaggttc ttctggtcct tggcactgat ctgttcatcc tcaggcacag cttccccgtt
      781 cctccacctg ttcagctcca cctccagatg ctggatgaca ctcttca
//
