LOCUS       sequence-102988-         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes the yeast Cwc25 protein for overexpression and purification 
            using a C-terminal hexahistidine tag. A single cysteine residue is 
            encoded after the Cwc25 sequence for fluorescent labeling..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Krishnan R, Blanco MR, Kahlscheuer ML, Abelson J, Guthrie C, Walter 
            NG
  TITLE     Biased Brownian ratcheting leads to pre-mRNA remodeling and capture 
            prior to first-step splicing.
  JOURNAL   Nat Struct Mol Biol. 2013 Dec;20(12):1450-7. doi: 10.1038/nsmb.2704.
            Epub 2013 Nov 17.
  PUBMED    24240612
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2704";
            journalName: "Nat Struct Mol Biol"; date: "2013-12"; volume: "20"; 
            issue: "12"; pages: "1450-7"
COMMENT     Sequence Label: Cwc25-pRSETA
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(38..55)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(56..76)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     RBS             639..661
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(692..711)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(693..711)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(796..813)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggctttgtta gcagccggat caagcttcga attcctaatg atgatgatga tgatgacctt
       61 gaaaataaag attttcacag ccgcctccgg cgccgtagtc taggtccgga gcgggcttgg
      121 acaacggttt ccctcgttgc gacattgctc ttttttttgt tgagtctgga gttcttcttt
      181 gttgtttagt taccttaaac ttactcattg gatcatcttt aagcaatttt gacttcttct
      241 tctgcgaact tatggatgtt gcggcaccag aagcggaaat agtagtggcc gcccttacag
      301 gcggtgttgc aggttggttt agtatagatg aatctagttt tttcttacct aataaatagt
      361 cttccttttc gtcactcagt tttgcatcct gatacatcca ttccaagccc gacttcttca
      421 aagccaagtc attcttgaga gtctccggct tatccttgct cgattcgttc agcagttcat
      481 ttagttctcg ttctttttca atttctttca atcgcgtgtt aagcttctgc tgttcggtaa
      541 ttagatcctg ttcggtctcc catacttttt ttctattttt cataagcttg ggattccatg
      601 atttcaacag attcaaatcg cccgacccca tatgtatatc tccttcttaa agttaaacaa
      661 aattatttct agagggaaac cgttgtggtc tccctatagt gagtcgtatt aatttcgcgg
      721 gatcgagatc ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
      781 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
      841 ggtatcagct cactcaa
//
