LOCUS       sequence-102996-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET-based biosensor for monitoring PKA activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Depry C, Allen MD, Zhang J
  TITLE     Visualization of PKA activity in plasma membrane microdomains.
  JOURNAL   Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10.1039/c0mb00079e. Epub 2010 
            Sep 14.
  PUBMED    20838685
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/c0mb00079e"; journalName: "Mol Biosyst"; date: "2011-01"; 
            volume: "7"; issue: "1"; pages: "52-8"
COMMENT     Sequence Label: pcDNA3-AKAR4
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(70..804)
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     primer_bind     263..282
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     522..543
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(649..670)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tatagaatag ggccctctag atgcatgctc gagcggccgc cagtgtgatg gatatctgca
       61 gaattcttac tcgatgttgt ggcggatctt gaagttggcc ttgatgccgt tcttctgctt
      121 gtcggcggtg atatagacgt tgtggctgtt gtagttgtac tccagcttgt gccccaggat
      181 gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc
      241 ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga agatggtgcg
      301 ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc
      361 ggggtagcgg gcgaagcact gaaggccgta gcccagggtg gtcacgaggg tgggccaggg
      421 cacgggcagc ttgccggtgg tgcagatcag cttcagggtc agcttgccgt aggtggcatc
      481 gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac
      541 caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatag atccaccggt
      601 acctcccttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag
      661 caggaccatg tgatcgcgct tctcgttggg gtctttgctc agcttggact ggtagctcag
      721 gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg
      781 gtcggcgagc tgcacgccgc ccatgagctc gctgccgccg gtgccgccgt ccacca
//
