LOCUS       sequence-102997-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Non-fluorescent, truncated kinesin-1 fused to DmrC linker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Norris SR, Soppina V, Dizaji AS, Schimert KI, Sept D, Cai D, 
            Sivaramakrishnan S, Verhey KJ
  TITLE     A method for multiprotein assembly in cells reveals independent 
            action of kinesins in complex.
  JOURNAL   J Cell Biol. 2014 Nov 10;207(3):393-406. doi: 10.1083/jcb.201407086.
            Epub 2014 Nov 3.
  PUBMED    25365993
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201407086"; journalName: "J Cell Biol"; date: 
            "2014-11-10- 10"; volume: "207"; issue: "3"; pages: "393-406"
COMMENT     Sequence Label: KIF5C(1-560)-DmrC
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(114..392)
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
ORIGIN
        1 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
       61 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatct agtctttgag
      121 attcgtcgga acacatgata atagaggtcc caggcttgga ggaggtcctt gacattccct
      181 gatttcatgt acttcctgca ccactcttgg gcctccatta aatctcgacc ataggcctga
      241 ttaaaggatg tttccttcag agtctggggg ccccgttcca tcatagcatg caagggctcc
      301 agcacctcaa acatgccttt cacgttcctt tccccaaagt acaaacgaga tgcctcttcc
      361 aggccttcat gccacatctc atgccagagg attctagatc cgcttcccac cggtcctcca
      421 cctcctcctc caccacctcc cggtaccagc tcgcccagat ccttgagaag caggttcagg
      481 atctccgtgg ccctcttctt ctggtggttg ctaagctcct gtagctgact cagctcccgc
      541 tgggtggttg tcagtgtcgt cgttttctgg gccagctcat cggtcagttg ctcgttggcc
      601 cgggtcttgt cctccacttc ctgtgacttc tggtcgtaat tgacagccag ctcctccagg
      661 gcctggagga cctccttcac ctcgtcttta gccgcctcgt tttcaatctg gaggcgggtc
      721 agctcctcct ggattttctc gtagtccctt ctggtggaag ctagaagctc gtcctgatcc
      781 aacatctgtt gcttcagctt ttcagccagc tggctctgct ggttaatttc atcgtccttg
      841 tcatccagct gtc
//
