LOCUS       sequence-102999-        1057 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Streptomyces expression of codon-optimized Cas9, tracrRNA, and 
            custom crRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Cobb RE, Wang Y, Zhao H
  TITLE     High-Efficiency Multiplex Genome Editing of Streptomyces Species 
            Using an Engineered CRISPR/Cas System.
  JOURNAL   ACS Synth Biol. 2014 Dec 8.
  PUBMED    25458909
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Dec 8."
COMMENT     Sequence Label: pCRISPomyces-1
FEATURES             Location/Qualifiers
     source          1..1057
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        491..851
                     /label=rpsL promoter
                     /note="promoter for the Cellulomonas flavigena ribosomal 
                     protein S12 gene"
     misc_RNA        852..930
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
     terminator      954..987
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 atctcggagt tcagcaagcg ggtcatcctg gccgacgcga acctcgacaa ggtgctgtcc
       61 gcctacaaca agcaccgcga caagccgatc cgggagcagg cggagaacat catccacctg
      121 ttcaccctca ccaacctggg cgcccccgcc gcgttcaagt acttcgacac caccatcgac
      181 cgcaagcggt acaccagcac caaggaggtc ctcgacgcga ccctgatcca ccagtccatc
      241 accggcctgt acgaaacccg catcgacctc tcccagctcg gcggcgactg agaattcaga
      301 tctacgcgtt ccccgcaaaa gcggcctttg actccctgca agcctcagcg accgaatata
      361 tcggttatgc gtgggcgatg gttgttgtca ttgtcggcgc aactatcggt atcaagctgt
      421 ttaagaaatt cacctcgaaa gcaagctgat aaaccgatac aattaaaggc tccttttgga
      481 gccttttttt ccgcccgcgg gacgtgacga gcggcacgac tcgacgactc cgggctcctt
      541 tgacgctgtc cgtcgcgccg ggtagcgtag gacaccgtgc ccgcgccgtc gggccctcgc
      601 gcgtgcactc ggtcgaccgc tccctgccgg agtgggtgcg ggtgcacggg gtggctcccc
      661 acctcctctc ggatcggtcc tcgcggactg ccgccgtgcg gaggaccggg gcgacacgcc
      721 cgggcgcggg ggtcggtgcg ggactccaga cctccggggt agtcgtgcga cgggcgacga
      781 tccgggccga gccggccgtc ctgggtgacg ggtgccggtc agaccagaga acaccgacag
      841 acggagacgt aggaaccatt caaaacagca tagcaagtta aaataaggct agtccgttat
      901 caacttgaaa aagtggcacc gagtcggtgc tttttttact ccatctggat ttgttcagaa
      961 cgctcggttg ccgccgggcg ttttttagct gctccttcgg tcggacgtgc gtctacgggc
     1021 accttaccgc agccgtcggc tgtgcgacac ggacgga
//
