LOCUS       sequence-10300-e        1929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1929)
  TITLE     PCRII-TOPO-ZFERV-ENV
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PCRII-TOPO-ZFERV-ENV
FEATURES             Location/Qualifiers
     source          1..1929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1845..1853
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 atgaatataa acaagatggt ggtggcctgg ggagtaatgt tggtgatgat cctatgggta
       61 atctgccata tggaattcgg aggaacaaca aaggaaaaac ggagtgtaaa agaagggggg
      121 ggaacagggg tgagtattac cttagaaagg gaagagggaa aggatgggat gtggcagttt
      181 gatctttgtc aagtgataga ttgtgggaag gaccaagtgg catggagaaa atatgatgtt
      241 tatgggtgtt tgtggccatc taccaccacg cttccgaatg gcccccattg tcatacctgg
      301 catgatgtaa attggaaaac agccccagga ttcactaaaa agatattgaa aaatagtcgt
      361 atggccagac cagaccacat ccaaaatcga cttaggttgt ctaggggata ccaacatagt
      421 tggggaggct ggaaaaacac gttgattata tcactaaaaa atggcaatga tgaaactgac
      481 acgtacatca ctctaggggt agatgtagaa ggaagagacc cactggggtt gataaaaatc
      541 tccataaaaa agcctaaacc cacaggggcg ccaataatca cagacctaac caaaaacaag
      601 aagaaagtaa tacagagcac tgactatagc aatctgaccc cactagatct gatgacgctg
      661 gagacaggct atcacgaaac aaatttatgg ttagaatggg tgacaaacgc agccgaagaa
      721 ttaggatttg aggggtgcct agcttgtgca gcaggaaggc cacaattaaa tactgaaccc
      781 gctccattac atgattatga tagttggggg tacaaatgta tgttaaaact aacaaaggaa
      841 aaaagtccca aaaactgcac tgcactcagt aatctatacc caccagctgg aaataagtca
      901 tcaatgggag ttgcattaaa aagaagaaag ggtaattata cctgtttcaa tttaactacc
      961 tccagtgcgt ccaaaatatt agtgggagcg tttaaaaggg aatggtgtgg acagatgatt
     1021 caggaaggcc atgacaaact agggggatgg ggcagagtgg gtttgtatta cgcgagtgga
     1081 gaaaaggtgg tattggctag aatagaaccc accatggaag gagtatgtgc tatgataaga
     1141 gtcgctgttc ctatggtcgt gataggaaat agggctataa atgggcataa aaacaggcga
     1201 aaaaggtcag taaatagtga tttcgattta acacgcaata gccccaccta catagacgca
     1261 ataggcatcc ccaggggagt tccagatgaa tataaattgg ctgaccaagt agcagcaggg
     1321 tttgaaaata tacctgtgat agcagcatta ttccccgtga ccccaaataa aaatgtagac
     1381 agaataaatt acatacatta caacgtacaa aggctgtcaa atctcactag agatgctgtg
     1441 tctggattaa aagaacaatt ggctgctacc tctctgatga ctattcaaaa tcggctagct
     1501 ttagacatgt tactatcaga aaggggcggg gtttgttcta tgtttaaaga cacgtgttgc
     1561 accgtcatac caaacaacac tgcccctgat ggttccgtat ccagggctct ggagggacta
     1621 aaagaattgt caaatgaact gaaagctagc tcagggatag aaaatgctgt gtcaaaatgg
     1681 atgtctgata tgtttgggga atggaaaggg gtgattattg ctatgttaac atcaatgggt
     1741 attttcctag gaattttggt gacatgtggg tgttgctgta ttccctgtat cagaagtttg
     1801 ataaataggc tgataataac tgcaattgaa aagaaggaaa acccacctcc ttatcagatg
     1861 cccctccttg ctgcagcgga gggggatgac gtgatggagg aagtggagga actgctggat
     1921 atggtgtga
//
