LOCUS       sequence-103001-         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Non-raft/plasma membrane-targeted FRET biosensor for monitoring PKA 
            activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Depry C, Allen MD, Zhang J
  TITLE     Visualization of PKA activity in plasma membrane microdomains.
  JOURNAL   Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10.1039/c0mb00079e. Epub 2010 
            Sep 14.
  PUBMED    20838685
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/c0mb00079e"; journalName: "Mol Biosyst"; date: "2011-01"; 
            volume: "7"; issue: "1"; pages: "52-8"
COMMENT     Sequence Label: pcDNA3-AKAR4-Kras
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(113..847)
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     primer_bind     306..325
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     565..586
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(692..713)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctatagaata gggccctcta gatgcatgct cgagcggccg ccagtgtgat ggatatctgc
       61 agaattctta catgatcacg cacttggtct tgctcttctt cttcttcttc ttctcgatgt
      121 tgtggcggat cttgaagttg gccttgatgc cgttcttctg cttgtcggcg gtgatataga
      181 cgttgtggct gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga
      241 agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg
      301 cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt
      361 cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cgggcgaagc
      421 actgaaggcc gtagcccagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg
      481 tggtgcagat cagcttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg
      541 acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc
      601 cggtgaacag ctcctcgccc ttgctcacca tagatccacc ggtacctccc ttgtacagct
      661 cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc
      721 gcttctcgtt ggggtctttg ctcagcttgg actggtagct caggtagtgg ttgtcgggca
      781 gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg agctgcacgc
      841 cgcccatgag ctcgctgccg ccggtgccgc cgtccacca
//
