LOCUS       sequence-103003-         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET-based biosensor for monitoring cAMP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   DiPilato LM, Zhang J
  TITLE     The role of membrane microdomains in shaping beta2-adrenergic 
            receptor-mediated cAMP dynamics.
  JOURNAL   Mol Biosyst. 2009 Aug;5(8):832-7. doi: 10.1039/b823243a. Epub 2009 
            May 26.
  PUBMED    19603118
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1039/b823243a"; 
            journalName: "Mol Biosyst"; date: "2009-08"; volume: "5"; issue: 
            "8"; pages: "832-7"
COMMENT     Sequence Label: pcDNA3-ICUE3
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(137..649,650..652,653..655))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     330..349
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     589..610
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(716..737)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctatagaata gggccctcta gatgcatgct cgagcggccg ccagtgtgat ggatatctgc
       61 agaattctta cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga
      121 gctgcacgcc gccgtcctcg atgttgtggc ggatcttgaa gttggccttg atgccgttct
      181 tctgcttgtc ggcggtgata tagacgttgt ggctgttgta gttgtactcc agcttgtgcc
      241 ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg
      301 tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga
      361 tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca
      421 tgtggtcggg gtagcgggcg aagcactgaa ggccgtagcc cagggtggtc acgagggtgg
      481 gccagggcac gggcagcttg ccggtggtgc agatcagctt cagggtcagc ttgccgtagg
      541 tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca
      601 gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc accatagatc
      661 caccggtacc tcccttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga
      721 actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagc ttggactggt
      781 agctcaggta gtggttgtcg ggcatgagct ctggctccag ctctcgggag aggcgggaga
      841 gttcccgctg gttgtcaatg accttcagct gctggacata agcccaggtg ctggctggac
      901 tccgggtgct c
//
