LOCUS       sequence-103014-         964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET-based biosensor for monitoring JNK activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Fosbrink M, Aye-Han NN, Cheong R, Levchenko A, Zhang J
  TITLE     Visualization of JNK activity dynamics with a genetically encoded 
            fluorescent biosensor.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Mar 23;107(12):5459-64. doi: 
            10.1073/pnas.0909671107. Epub 2010 Mar 8.
  PUBMED    20212108
REFERENCE   2  (bases 1 to 964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0909671107"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2010-03-23- 23"; volume: "107"; issue: "12"; pages: "5459-64"
COMMENT     Sequence Label: pcDNA3-JNKAR1
FEATURES             Location/Qualifiers
     source          1..964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(68..781,782..784,785..787))
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(113..134)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     462..481
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     721..742
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ctatagaata gggccctcta gatgcatgct cgagcggccg ccagtgtgat ggatatctgc
       61 agaattctta cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact
      121 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagcttg gactggtagc
      181 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt
      241 agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct
      301 tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact
      361 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc
      421 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt
      481 ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt
      541 cgtgctgctt catgtggtcg gggtagcggg cgaagcacat caggccgtag ccgaaggtgg
      601 tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca
      661 gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta
      721 cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc
      781 tcaccatgag ctcgctgccg ccggtgccgc ccttcttctc gagctgctcg agcagtgggt
      841 tgccttcatc ctcgggggtc ttgacactgt cgaccaggcc cttggtgctg ccctcgccgc
      901 tgcctggctt acctgcagag cgatcaactt tgttctgctc gaggcactgc ttaaatttgt
      961 cgtt
//
