LOCUS       sequence-103023-        1036 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Streptomyces expression of codon-optimized Cas9, tracrRNA, and 
            custom crRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Cobb RE, Wang Y, Zhao H
  TITLE     High-Efficiency Multiplex Genome Editing of Streptomyces Species 
            Using an Engineered CRISPR/Cas System.
  JOURNAL   ACS Synth Biol. 2014 Dec 8.
  PUBMED    25458909
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Dec 8."
COMMENT     Sequence Label: pCRISPomyces-1
FEATURES             Location/Qualifiers
     source          1..1036
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agaagctggt cgactcgacc gacaaggcgg acctgcggct catctacctg gccctcgcgc
       61 acatgatcaa gttccgcggc cacttcctca tcgagggcga cctgaacccg gacaactccg
      121 acgtcgacaa gctcttcatc cagctggtgc agacctacaa ccagctgttc gaggagaacc
      181 ccatcaacgc cagcggcgtc gacgccaagg cgatcctctc cgcgcgcctg agcaagtccc
      241 ggcgcctgga gaacctcatc gcccagctgc cgggcgagaa gaagaacggc ctcttcggca
      301 acctgatcgc gctgtcgctc ggcctgaccc ccaacttcaa gagcaacttc gacctggccg
      361 aggacgcgaa gctccagctg tccaaggaca cctacgacga cgacctggac aacctgctcg
      421 cccagatcgg cgaccagtac gcggacctct tcctggccgc gaagaacctc tcggacgcca
      481 tcctgctcag cgacatcctg cgggtcaaca ccgagatcac caaggccccg ctgtcggcga
      541 gcatgatcaa gcggtacgac gagcaccacc aggacctgac cctgctcaag gccctcgtgc
      601 gccagcagct gcccgagaag tacaaggaga tcttcttcga ccagtccaag aacggctacg
      661 ccggctacat cgacggcggc gcgtcgcagg aggagttcta caagttcatc aagccgatcc
      721 tggagaagat ggacggcacc gaggagctgc tcgtcaagct gaaccgcgag gacctgctcc
      781 gcaagcagcg gaccttcgac aacggctcca tcccgcacca gatccacctg ggcgagctcc
      841 acgccatcct ccggcgccag gaggacttct accccttcct gaaggacaac cgcgagaaga
      901 tcgagaagat cctgaccttc cggatcccgt actacgtcgg ccccctggcc cgcggcaact
      961 cccggttcgc gtggatgacc cggaagtcgg aggaaaccat caccccgtgg aacttcgagg
     1021 aggtcgtgga caaggg
//
