LOCUS       sequence-103024-         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Streptomyces expression of codon-optimized Cas9, tracrRNA, and 
            custom crRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Cobb RE, Wang Y, Zhao H
  TITLE     High-Efficiency Multiplex Genome Editing of Streptomyces Species 
            Using an Engineered CRISPR/Cas System.
  JOURNAL   ACS Synth Biol. 2014 Dec 8.
  PUBMED    25458909
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Dec 8."
COMMENT     Sequence Label: pCRISPomyces-1
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cgagggcatg cggaagccgg ccttcctgtc cggcgagcag aagaaggcga tcgtcgacct
       61 gctcttcaag accaaccgca aggtcaccgt gaagcagctg aaggaggact acttcaagaa
      121 gatcgagtgc ttcgactccg tcgagatctc gggcgtggag gaccgcttca acgcctccct
      181 gggcacctac cacgacctgc tcaagatcat caaggacaag gacttcctcg acaacgagga
      241 gaacgaggac atcctggagg acatcgtcct caccctgacc ctcttcgagg accgcgagat
      301 gatcgaggag cggctcaaga cctacgccca cctgttcgac gacaaggtga tgaagcagct
      361 gaagcggcgc cggtacaccg gctggggccg cctctcccgg aagctgatca acggcatccg
      421 ggacaagcag agcggcaaga ccatcctgga cttcctcaag tccgacggct tcgccaaccg
      481 caacttcatg cagctcatcc acgacgactc gctgaccttc aaggaggaca tccagaaggc
      541 ccaggtgtcc ggccagggcg acagcctcca cgagcacatc gccaacctgg cgggctcccc
      601 ggcgatcaag aagggcatcc tccagaccgt caaggtcgtg gacgagctgg tcaaggtgat
      661 gggccgccac aagcccgaga acatcgtgat cgagatggcc cgggagaacc agaccaccca
      721 gaagggccag aagaactccc gcgagcggat gaagcgcatc gaggagggca tcaaggagct
      781 cggctcgcag atcctgaagg agcacccggt cgagaacacc cagctccaga acgagaagct
      841 gtacctctac tacctgcaga acggccgcga catgtacgtg gaccaggagc tcgacatcaa
      901 ccggctgagc gactacgacg tcgaccacat
//
