LOCUS       sequence-103034-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian Expression of Venus-oLID-CAAX .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Guntas G, Hallett RA, Zimmerman SP, Williams T, Yumerefendi H, Bear 
            JE, Kuhlman B
  TITLE     Engineering an improved light-induced dimer (iLID) for controlling 
            the localization and activity of signaling proteins.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Jan 6;112(1):112-7. doi: 
            10.1073/pnas.1417910112. Epub 2014 Dec 22.
  PUBMED    25535392
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1417910112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-01-6- 6"; volume: "112"; issue: "1"; pages: "112-7"
COMMENT     Sequence Label: pLL7.0: Venus-oLID-CAAX (from KRas4B)
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      25..34
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(31..33,34..36,37..747)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(76..97)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(337..356)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     684..705
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccgcgggccc gggatccacc ggtcgccacc atggtgagca agggcgagga gctgttcacc
       61 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
      121 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt gatctgcacc
      181 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctcggcta cggcctgcag
      241 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
      301 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
      361 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
      421 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
      481 gtctatatca ccgccgacaa gcagaagaac ggcatcaagg caaacttcaa gatccgccac
      541 aacatcgagg acggcggcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
      601 gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccaa actgagcaaa
      661 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
      721 actctcggca tggacgagct gtacaagggt agtggtagtg gggagtttct ggcaaccaca
      781 ctggaacgga tcgag
//
