LOCUS       sequence-103038-         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian Expression of Venus-oLID-CAAX .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Guntas G, Hallett RA, Zimmerman SP, Williams T, Yumerefendi H, Bear 
            JE, Kuhlman B
  TITLE     Engineering an improved light-induced dimer (iLID) for controlling 
            the localization and activity of signaling proteins.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Jan 6;112(1):112-7. doi: 
            10.1073/pnas.1417910112. Epub 2014 Dec 22.
  PUBMED    25535392
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1417910112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-01-6- 6"; volume: "112"; issue: "1"; pages: "112-7"
COMMENT     Sequence Label: pLL7.0: Venus-oLID-CAAX (from KRas4B)
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    99..132
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(724..745)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 taccagtcaa tctttcacaa attttgtaat ccagaggttg attatcgata agcttgatat
       61 cgaattgtac ctagtggaac cggaaccctt aaacatgtat aacttcgtat aatgtatgct
      121 atacgaagtt attaggtccc tcgacgaatt gcctgcaggc tacataatta cacactttgt
      181 ctttgacttc tttttcttct ttttgctccc actcccgctt ccaaagtaat tttcgtcgtt
      241 cgctgcctca tcaatttctt cggctgtctt tttgatcagc atgaccgcct cacgttcagc
      301 ggcgtcgcga acatgttcgg tgccatccag ctgcacgcca atgaagtact ggacgtcacc
      361 tttctgatcg cgcatcggct gcaggtggaa caggttccaa aactttttgc cgctcttggt
      421 ataattgatc agctgaacgg tcacttcagt ctggttgtca atagcatctc tgattttccg
      481 aacagtggca cggtctgtct cgggtccctg cagaaaacgg caattgcgcc ccaggatctc
      541 ttcccgagaa tattctgtca gctgcaggaa ggaatcgctc gcaaaaatga ttgggttgtc
      601 aggcagtctc ggatcagtaa tcacgaaatt tttctcgatc cgttccagtg tggttgccag
      661 aaactcccca ctaccactac ccttgtacag ctcgtccatg ccgagagtga tcccggcggc
      721 ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagttt
      781 ggactgg
//
