LOCUS       sequence-103047-         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tandem mCitrine-labeled, truncated kinesin-1 fused to split GFP 
            linker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Norris SR, Soppina V, Dizaji AS, Schimert KI, Sept D, Cai D, 
            Sivaramakrishnan S, Verhey KJ
  TITLE     A method for multiprotein assembly in cells reveals independent 
            action of kinesins in complex.
  JOURNAL   J Cell Biol. 2014 Nov 10;207(3):393-406. doi: 10.1083/jcb.201407086.
            Epub 2014 Nov 3.
  PUBMED    25365993
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201407086"; journalName: "J Cell Biol"; date: 
            "2014-11-10- 10"; volume: "207"; issue: "3"; pages: "393-406"
COMMENT     Sequence Label: KIF5C(1-560)-2xmCitrine-GFP11
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(117..164)
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
ORIGIN
        1 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
       61 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatct agatcaggtg
      121 atgccggcgg cgttcacgta ctcgtgcagc accatgtggt ctctgccgct gcctaccggt
      181 gaacctcctg gcgcgccctt gtacagctcg tccatgccca gagtaatccc tgcggctgtc
      241 acaaactcaa gcagcaccat atgatcccgc ttttcgttag ggtccttgct gagtttgctc
      301 tgatagctga ggtaatggtt atcggggagc aggactggac cgtccccgat tggggtattc
      361 tgttggtaat ggtctgcgag ttgcacactg ccatcctcaa tgttatggcg tatcttaaag
      421 ttgaccttaa tgccattctt ttgcttatcg gccatgatgt agacattgtg actgttatag
      481 ttatactcga gcttatgtcc gaggatattg ccatcttctt tgaaatcgat ccccttgagc
      541 tctatgcgat tcacgagggt atcgccttcg aatttcacct cggcccgggt tttgtaattg
      601 ccatcgtcct tgaagaagat tgtgcgttcc tgcacgtacc cttcaggcat tgcggattta
      661 aaaaagtcat gctgtttcat atggtcaggg taacgggcaa agcacatcag cccgtacccg
      721 aatgtggtga cgagtgtggg ccaggggacg gggagcttcc cggttgtgca tatgaatttc
      781 agagtcagtt tgccataggt tgcatcccct tca
//
