LOCUS       sequence-103053-         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Streptomyces expression of codon-optimized Cas9 and custom gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Cobb RE, Wang Y, Zhao H
  TITLE     High-Efficiency Multiplex Genome Editing of Streptomyces Species 
            Using an Engineered CRISPR/Cas System.
  JOURNAL   ACS Synth Biol. 2014 Dec 8.
  PUBMED    25458909
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Dec 8."
COMMENT     Sequence Label: pCRISPomyces-2
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        481..765
                     /label=gapdh promoter
                     /note="glyceraldehyde-3-phosphate dehydrogenase promoter 
                     from Eggerthella lenta"
     primer_bind     920..942
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     934..951
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     935..951
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     961..980
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        961..979
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tcagcaagcg ggtcatcctg gccgacgcga acctcgacaa ggtgctgtcc gcctacaaca
       61 agcaccgcga caagccgatc cgggagcagg cggagaacat catccacctg ttcaccctca
      121 ccaacctggg cgcccccgcc gcgttcaagt acttcgacac caccatcgac cgcaagcggt
      181 acaccagcac caaggaggtc ctcgacgcga ccctgatcca ccagtccatc accggcctgt
      241 acgaaacccg catcgacctc tcccagctcg gcggcgactg agaattcaga tctacgcgtt
      301 ccccgcaaaa gcggcctttg actccctgca agcctcagcg accgaatata tcggttatgc
      361 gtgggcgatg gttgttgtca ttgtcggcgc aactatcggt atcaagctgt ttaagaaatt
      421 cacctcgaaa gcaagctgat aaaccgatac aattaaaggc tccttttgga gccttttttt
      481 gctgctcctt cggtcggacg tgcgtctacg ggcaccttac cgcagccgtc ggctgtgcga
      541 cacggacgga tcgggcgaac tggccgatgc tgggagaagc gcgctgctgt acggcgcgca
      601 ccgggtgcgg agcccctcgg cgagcggtgt gaaacttctg tgaatggcct gttcggttgc
      661 tttttttata cggctgccag ataaggcttg cagcatctgg gcggctaccg ctatgatcgg
      721 ggcgttcctg caattcttag tgcgagtatc tgaaagggga tacgcaagtc ttctcagccg
      781 ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
      841 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
      901 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg
      961 taatacgact cactataggg cgaattgggt accggg
//
