LOCUS       sequence-103058-         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Streptomyces expression of codon-optimized Cas9 and custom gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Cobb RE, Wang Y, Zhao H
  TITLE     High-Efficiency Multiplex Genome Editing of Streptomyces Species 
            Using an Engineered CRISPR/Cas System.
  JOURNAL   ACS Synth Biol. 2014 Dec 8.
  PUBMED    25458909
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Dec 8."
COMMENT     Sequence Label: pCRISPomyces-2
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agggcatgcg gaagccggcc ttcctgtccg gcgagcagaa gaaggcgatc gtcgacctgc
       61 tcttcaagac caaccgcaag gtcaccgtga agcagctgaa ggaggactac ttcaagaaga
      121 tcgagtgctt cgactccgtc gagatctcgg gcgtggagga ccgcttcaac gcctccctgg
      181 gcacctacca cgacctgctc aagatcatca aggacaagga cttcctcgac aacgaggaga
      241 acgaggacat cctggaggac atcgtcctca ccctgaccct cttcgaggac cgcgagatga
      301 tcgaggagcg gctcaagacc tacgcccacc tgttcgacga caaggtgatg aagcagctga
      361 agcggcgccg gtacaccggc tggggccgcc tctcccggaa gctgatcaac ggcatccggg
      421 acaagcagag cggcaagacc atcctggact tcctcaagtc cgacggcttc gccaaccgca
      481 acttcatgca gctcatccac gacgactcgc tgaccttcaa ggaggacatc cagaaggccc
      541 aggtgtccgg ccagggcgac agcctccacg agcacatcgc caacctggcg ggctccccgg
      601 cgatcaagaa gggcatcctc cagaccgtca aggtcgtgga cgagctggtc aaggtgatgg
      661 gccgccacaa gcccgagaac atcgtgatcg agatggcccg ggagaaccag accacccaga
      721 agggccagaa gaactcccgc gagcggatga agcgcatcga ggagggcatc aaggagctcg
      781 gctcgcagat cctgaaggag cacccggtcg agaacaccca gctccagaac gagaagctgt
      841 acctctacta cctgcagaac ggccgcgaca tgtacgtgga ccaggagctc gacatcaacc
      901 ggctgagcga ctacgacgtc gaccacatcg tgccgcagtc cttcc
//
