LOCUS       sequence-10306-e        4792 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Lawson ND, Vogel AM, Weinstein BM
  TITLE     sonic hedgehog and vascular endothelial growth factor act upstream 
            of the Notch pathway during arterial endothelial differentiation.
  JOURNAL   Dev Cell. 2002 Jul . 3(1):127-36.
  PUBMED    12110173
REFERENCE   2  (bases 1 to 4792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2002 Jul . 3(1):127-36."
COMMENT     Sequence Label: pCS2vegf165
FEATURES             Location/Qualifiers
     source          1..4792
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     polyA_signal    841..975
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(865..884)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     919..938
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1088..1108)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1088..1106)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1127..1143)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1127..1143)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1140..1162)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1151..1167
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1175..1181,1182..1199,1200..1205))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1220..1241
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1358..1375)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1529..2117)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1609..1628)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2288..3079,3080..3148))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2911..2930
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3149..3253)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3279..3734)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3416..3437)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3628..3647
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3808..4792
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atccacaatc atgaacttgg ttgtttattt gatacagtta
      121 tttctcgcgg ctctcctcca tctgtctgct gtaaaggctg cccacatacc caaagaaggg
      181 ggaaagagca aaaatgatgt gattcccttc atggatgtgt ataaaaagag tgcgtgcaag
      241 acccgagagc tgctggtaga catcatccag gagtatcccg atgagatcga gcacacgtac
      301 atcccgtcct gtgtggttct catgcgctgt gcaggatgct gtaatgatga ggcgctcgaa
      361 tgcgtcccga cagagacacg aaacgtcact atggaggtgc tgcgggtcaa gcaacgcgta
      421 tcgcagcata attttcagct gagtttcaca gaacacacca agtgtgaatg caggccaaag
      481 gcagaagtca aagcaaagaa agaaaaccac tgtgagcctt gctcagagag aaggaagcgc
      541 ttgtatgtgc aggaccccct cacctgtaaa tgctcctgca aattcacaca aatgcaatgc
      601 aagtccagac aacttgagtt aaacgaaaga acttgcagat gtgaaaagcc aagatgatga
      661 tcgagcgaac gccgggcaga actctgcatg aaggaaggaa cttaaataaa aactttttaa
      721 acggcacagc actttcaaaa cctaaggaag aacattcctt ggatgaactg caaacactat
      781 cctaaacgaa acactaagtg cagctcgagc ctctagaact atagtgagtc gtattacgta
      841 gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
      901 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
      961 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     1021 gtgtgggagg ttttttaatt cgcggccgcg gcgccaatgc attgggcccg gtacccagct
     1081 tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc
     1141 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     1201 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     1261 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     1321 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     1381 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     1441 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     1501 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     1561 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     1621 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     1681 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     1741 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     1801 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     1861 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     1921 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     1981 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     2041 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     2101 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     2161 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     2221 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     2281 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     2341 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     2401 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     2461 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     2521 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     2581 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     2641 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     2701 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     2761 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     2821 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     2881 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     2941 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     3001 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     3061 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     3121 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     3181 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     3241 taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt
     3301 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     3361 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     3421 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     3481 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     3541 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     3601 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     3661 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     3721 gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     3781 gcgggcctct tcgctattac gccagtcgac catagccaat tcaatatggc gtatatggac
     3841 tcatgccaat tcaatatggt ggatctggac ctgtgccaat tcaatatggc gtatatggac
     3901 tcgtgccaat tcaatatggt ggatctggac cccagccaat tcaatatggc ggacttggca
     3961 ccatgccaat tcaatatggc ggacttggca ctgtgccaac tggggagggg tctacttggc
     4021 acggtgccaa gtttgaggag gggtcttggc cctgtgccaa gtccgccata ttgaattggc
     4081 atggtgccaa taatggcggc catattggct atatgccagg atcaatatat aggcaatatc
     4141 caatatggcc ctatgccaat atggctattg gccaggttca atactatgta ttggccctat
     4201 gccatatagt attccatata tgggttttcc tattgacgta gatagcccct cccaatgggc
     4261 ggtcccatat accatatatg gggcttccta ataccgccca tagccactcc cccattgacg
     4321 tcaatggtct ctatatatgg tctttcctat tgacgtcata tgggcggtcc tattgacgta
     4381 tatggcgcct cccccattga cgtcaattac ggtaaatggc ccgcctggct caatgcccat
     4441 tgacgtcaat aggaccaccc accattgacg tcaatgggat ggctcattgc ccattcatat
     4501 ccgttctcac gccccctatt gacgtcaatg acggtaaatg gcccacttgg cagtacatca
     4561 atatctatta atagtaactt ggcaagtaca ttactattgg aaggacgcca gggtacattg
     4621 gcagtactcc cattgacgtc aatggcggta aatggcccgc gatggctgcc aagtacatcc
     4681 ccattgacgt caatggggag gggcaatgac gcaaatgggc gttccattga cgtaaatggg
     4741 cggtaggcgt gcctaatggg aggtctatat aagcaatgct cgtttaggga ac
//
