LOCUS       sequence-103061-         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  10nm SAH scaffold with EF Hand and split GFP attachment sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Norris SR, Soppina V, Dizaji AS, Schimert KI, Sept D, Cai D, 
            Sivaramakrishnan S, Verhey KJ
  TITLE     A method for multiprotein assembly in cells reveals independent 
            action of kinesins in complex.
  JOURNAL   J Cell Biol. 2014 Nov 10;207(3):393-406. doi: 10.1083/jcb.201407086.
            Epub 2014 Nov 3.
  PUBMED    25365993
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201407086"; journalName: "J Cell Biol"; date: 
            "2014-11-10- 10"; volume: "207"; issue: "3"; pages: "393-406"
COMMENT     Sequence Label: SNAP-EF(N)-10nmSAH-GFP(1-10)
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             70..711
                     /codon_start=1
                     /product="fragment of superfolder GFP consisting of the 
                     first 10 beta-strands"
                     /label=GFP(1-10)
                     /note="associates with GFP11, the 11th beta-strand, to 
                     reconstitute fluorescent GFP (Cabantous et al., 2005; 
                     Kamiyama et al., 2016)"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK"
ORIGIN
        1 gaagagagaa agaaaaggga cgatgatgaa aaacgtggaa gcggaggaag cggaaagctt
       61 ggatccggaa tgtccaaagg agaagaactg tttaccggtg ttgtgccaat tttggttgaa
      121 ctcgatggtg atgtcaacgg acataagttc tcagtgagag gcgaaggaga aggtgacgcc
      181 accattggaa aattgactct taaattcatc tgtactactg gtaaacttcc tgtaccatgg
      241 ccgactctcg taacaacgct tacgtacgga gttcagtgct tttcgagata cccagaccat
      301 atgaaaagac atgacttttt taagtcggct atgcctgaag gttacgtgca agaaagaaca
      361 atttcgttca aagatgatgg aaaatataaa actagagcag ttgttaaatt tgaaggagat
      421 actttggtta accgcattga actgaaagga acagatttta aagaagatgg taatattctt
      481 ggacacaaac tcgaatacaa ttttaatagt cataacgtat acatcactgc tgataagcaa
      541 aagaacggaa ttaaagcgaa tttcacagta cgccataatg tagaagatgg cagtgttcaa
      601 cttgccgacc attaccaaca aaacacccct attggagacg gtccggtact tcttcctgat
      661 aatcactacc tctcaacaca aacagtcctg agcaaagatc caaatgaaaa aggaacagtc
      721 gacggtaccg cgggcccggg atccaccgga tctagataac tgatcataat cagccatacc
      781 aca
//
