LOCUS       sequence-103064-        9137 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ePathBrick pETM6 vector encoding CsF3H, FaDFR, and DuLAR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9137)
  AUTHORS   Zhao S, Jones JA, Lachance DM, Bhan N, Khalidi O, Venkataraman S, 
            Wang Z, Koffas MA
  TITLE     Improvement of catechin production in Escherichia coli through 
            combinatorial metabolic engineering.
  JOURNAL   Metab Eng. 2014 Dec 17;28C:43-53. doi: 10.1016/j.ymben.2014.12.002.
  PUBMED    25527438
REFERENCE   2  (bases 1 to 9137)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2014-12-17"; volume: "28C:43-53. doi"; pages: " 
            10.1016/j.ymben.2014.12.002"
COMMENT     Sequence Label: p168
FEATURES             Location/Qualifiers
     source          1..9137
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             219..230
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1287..1331
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      1384..1431
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        1465..1483
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1484..1508
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1523..1545
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2624..2668
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      2721..2768
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        2802..2820
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2821..2845
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2860..2882
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4072..4116
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      4169..4216
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      4260..4715
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     4557..4578
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(4830..5621,5622..5690))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5453..5472
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5691..5783)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5864..6452
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6353..6372
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6606..6623
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6638..6780
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6706..6725)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6866..6888
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6882..7073)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(7645..8727)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     8689..8708
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(8728..8805)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     9011..9030
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        join(9121..9137,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatggcaccg accaccaccc tgaccgcact ggcagaagaa aaaagcctgc
      121 agcagaaatt tgttcgtgat gaagatgaac gtccgaaagt tgcctataat gtgtttagca
      181 atgaaatccc ggttattagc ctggcaggta ttgatgaaat tgaaggtcgt cgtagcgaaa
      241 tttgccgtaa aattgttgaa gcatgtgaag gttggggtgt ttttcaggtt gttgatcatg
      301 gtgttgatgc aaatctgatt gcagaaatga cccgtctggc acgtgaattt tttgcactgc
      361 ctccggaaga aaaactgcgt tttgatatga gcggtggtaa aaaaggtggt tttattgtta
      421 gcagccatct gcagggtgaa gcagttcagg attggcgtga aattgttacc tatttcagct
      481 atccgattcg tgcacgtgat tatagccgtt ggcctgataa accggaaggt tggcgtgcag
      541 ttaccgaaac ctatagcgaa aaactgatgg atctggcatg taaactgctg gaagttctga
      601 gcgaagcaat gggtctggaa aaagaggcac tgaccaaagc atgtgttgat atggatcaga
      661 aagtggtgat caacttctat ccgaaatgtc cgcagccgga tctgaccctg ggtctgaaac
      721 gtcataccga tccgggtaca attaccctgc tgctgcaaga tcaggtgggt ggtctgcagg
      781 caacccgtga tggtggcaaa acctggatta ccgttcagcc ggttgaaggt gcatttgttg
      841 ttaatctggg tgatcatggc cattatctga gcaatggtcg ctttaaaaac gcagatcatc
      901 aggcagttgt taatagcaat tgtagccgtc tgagcattgc aacctttcag aatccggcac
      961 cggaagcaac cgtttatccg ctgaaaattc gtgaaggtga aaaaccgatt ctggaagaac
     1021 cgattacctt tgccgatatg tataaacgca aaatgagcaa agatatcgag ctggccaaac
     1081 tgaaaaaact ggcgaaagaa aaaaaactgc tgcaagacca gcaggatatc gaaaaagcaa
     1141 aactggaaat caaaagcacc gatgaaatct tcgccctggt tggtgcactg atgcatgtta
     1201 tgcagaaacg tagccgtgca attcatagca gtgatgaagg tgaagatcaa gccggtgatg
     1261 aagatgagga ttaactcgag tctggtaaag aaaccgctgc tgcgaaattt gaacgccagc
     1321 acatggactc gtctactagt cgcagcttaa ttaacctaaa ctgctgccac cgctgagcaa
     1381 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctaggatcg
     1441 agatcgatct cgatcccgcg aaattaatac gactcactat aggggaattg tgagcggata
     1501 acaattcccc tctagaaata attttgttta actttaagaa ggagatatac atatgggtct
     1561 gggtgcagaa agcggtagcg tttgtgttac cggtgcaagc ggttttgttg gtagctggct
     1621 ggttatgcgt ctgctggaac atggttatac cgttcgtgca accgtgcgtg atccggcaaa
     1681 tctgaaaaaa gttcgtcatc tgctggaact gccgcaggca gcaacccgtc tgaccctgtg
     1741 gaaagcagat ctggatgttg aaggtagctt tgatgaagcc attaaaggtt gtaccggtgt
     1801 ttttcatgtt gcaaccccga tggattttga aagcgaagat ccggaaaacg aagttattaa
     1861 accgaccatt aacggcatgc tggatattat gaaagcatgc ctgaaagcaa aaaccgttcg
     1921 tcgtctggtt tttaccagca gtgccggtgc agttgcaatt gaagaacatc cgaaagaagt
     1981 gtacagcgaa aataactggt cagatgttgt gttttgccgc aaagttaaaa tgaccggctg
     2041 gatgtatttt gtgagcaaaa ccctggcaga acaggcagca tggaaatttg caaaagaaaa
     2101 caacatcgac ttcatcacca ttattccgac cctggttatt ggtccgtttc tggcaccgag
     2161 catgcctccg agcctgatta gcggtctgag tccgctgacc ggtaatgaag cacattatgg
     2221 tattatcaaa cagtgccagt atgtgcatct ggatgatctg tgtcagagcc atatttttct
     2281 gtatgaacat gcaaaagccg agggtcgtta tatttgtagc agccatgatg caaccattca
     2341 cgatattgca aaactgctga acgagaaata cccgaaatac aacgttccga aaaaattcaa
     2401 aggcatcgaa gaaaacctga ccaacattca ctttagcagc aaaaaactga aagagatggg
     2461 cttcgaattt aaacacagcc tggaagatat gtttacaggt gccgttgatg catgtcgtga
     2521 aaaaggtctg ctgccgctgc cgcaagaaga agaaaccgaa aaacgtcgtg caggtcctcc
     2581 tcctgcactg cctccgaaac gtcgtcgtta actcgagtct ggtaaagaaa ccgctgctgc
     2641 gaaatttgaa cgccagcaca tggactcgtc tactagtcgc agcttaatta acctaaactg
     2701 ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg
     2761 gttttttgct aggatcgaga tcgatctcga tcccgcgaaa ttaatacgac tcactatagg
     2821 ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact ttaagaagga
     2881 gatatacata tgaccgttag cggtgcaatt ccgagcatga ccaaaaatcg taccctggtt
     2941 gttggtggca ccggttttat tggtcagttt attaccaaag caagcctggg ttttggttat
     3001 ccgacctttc tgctggttcg tccgggtccg gttagcccga gcaaagcagt tattatcaaa
     3061 acctttcagg ataaaggtgc caaagtgatt tatggcgtga tcaacgataa agaatgcatg
     3121 gaaaaaattc tgaaagagta cgagatcgac gttgttatta gcctggtggg tggtgcacgt
     3181 ctgctggatc agctgaccct gctggaagca attaaaagcg ttaaaaccat caaacgtttt
     3241 ctgccgagcg aatttggcca tgatgttgat cgtaccgatc cggttgaacc gggtctgacc
     3301 atgtataaag aaaaacgtct ggtgcgtcgt gccgttgaag aatatggtat tccgtttacc
     3361 aatatctgct gcaatagcat tgcaagctgg ccgtattatg ataattgtca tccgagccag
     3421 gttccgcctc cgatggatca gtttcagatt tatggtgatg gtaacaccaa agcctatttc
     3481 attgatggca acgatatcgg caaatttacc atgaaaacca tcgatgatat tcgcaccctg
     3541 aacaaaaatg ttcattttcg tccgagcagc aactgctaca gcattaatga actggcaagc
     3601 ctgtgggaga aaaaaatcgg tcgtacactg cctcgtttta ccgttaccgc agataaactg
     3661 ctggcacatg cagcagaaaa cattattccg gaaagcattg ttagcagctt tacccacgat
     3721 atctttatta acggttgcca ggtgaacttt agcatcgatg aacatagtga tgtggaaatc
     3781 gatacactgt atccggatga aaaatttcgt agcctggatg attgctatga agattttgtt
     3841 ccgatggtgc acgataaaat tcatgcaggt aaaagcggtg aaatcaaaat caaagatggt
     3901 aaaccgctgg ttcagaccgg caccattgaa gaaattaaca aagacattaa aaccctggtg
     3961 gaaacccagc cgaatgaaga gatcaaaaaa gatatgaaag cactggttga agccgttccg
     4021 attagcgcaa tgggtggtgt taaagaaagc ctggtttaac tcgagtctgg taaagaaacc
     4081 gctgctgcga aatttgaacg ccagcacatg gactcgtcta ctagtcgcag cttaattaac
     4141 ctaaactgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt
     4201 cttgaggggt tttttgctag cgaaaggagg agtcgactat atccggattg gcgaatggga
     4261 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     4321 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     4381 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     4441 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     4501 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     4561 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     4621 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     4681 cgcgaatttt aacaaaatat taacgtttac aatttctggc ggcacgatgg catgagatta
     4741 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     4801 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     4861 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     4921 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     4981 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     5041 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     5101 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     5161 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     5221 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     5281 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     5341 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     5401 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     5461 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     5521 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     5581 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     5641 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     5701 tttcaatcat gattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     5761 atgtatttag aaaaataaac aaataggtca tgaccaaaat cccttaacgt gagttttcgt
     5821 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     5881 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     5941 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     6001 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     6061 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     6121 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     6181 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     6241 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     6301 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     6361 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     6421 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     6481 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     6541 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     6601 agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta
     6661 cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga
     6721 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
     6781 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     6841 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     6901 tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca
     6961 cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc
     7021 tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc
     7081 tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg
     7141 ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa
     7201 caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc
     7261 ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc
     7321 cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa
     7381 ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac
     7441 gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc
     7501 cgggtcctca acgacaggag cacgatcatg ctagtcatgc cccgcgccca ccggaaggag
     7561 ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc
     7621 taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     7681 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag
     7741 ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc
     7801 ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt
     7861 gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac
     7921 cgagatgtcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc
     7981 catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat
     8041 ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat
     8101 ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact
     8161 taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc
     8221 cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac
     8281 atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc
     8341 atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc
     8401 cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag
     8461 ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact
     8521 ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt
     8581 gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac
     8641 gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc
     8701 gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg
     8761 ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct
     8821 ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca
     8881 ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg
     8941 ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat
     9001 cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga
     9061 tgccggccac gatgcgtccg gcgtagccta ggatcgagat cgatctcgat cccgcgaaat
     9121 taatacgact cactata
//
