LOCUS       sequence-103065-         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian Expression of Venus-iLID-Mito.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Guntas G, Hallett RA, Zimmerman SP, Williams T, Yumerefendi H, Bear 
            JE, Kuhlman B
  TITLE     Engineering an improved light-induced dimer (iLID) for controlling 
            the localization and activity of signaling proteins.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Jan 6;112(1):112-7. doi: 
            10.1073/pnas.1417910112. Epub 2014 Dec 22.
  PUBMED    25535392
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1417910112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-01-6- 6"; volume: "112"; issue: "1"; pages: "112-7"
COMMENT     Sequence Label: pLL7.0: Venus-iLID-Mito (From ActA)
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    99..132
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(322..759)
                     /codon_start=1
                     /product="improved light-inducible dimer when used with 
                     SspB nano or SspB micro (Guntas et al., 2015)"
                     /label=iLID
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNVFHLQPMRDYKGDVQ
                     YFIGVQLDGTERLHGAAEREAVCLIKKTAFQIAEAANDENYF"
ORIGIN
        1 taccagtcaa tctttcacaa attttgtaat ccagaggttg attatcgata agcttgatat
       61 cgaattgtac ctagtggaac cggaaccctt aaacatgtat aacttcgtat aatgtatgct
      121 atacgaagtt attaggtccc tcgacgaatt gcctgcaggt taattatttt ttcttaattg
      181 aataattttg ataaacgccc ctaaagagaa cacgccaata gctaacattg caagaattac
      241 gatcctatgg ttccctggtt cttccttcgc tttttcgtct tctgcacttt tagcaattag
      301 tttgctccca ctcccgcttc caaagtaatt ttcgtcgttc gctgcctcag caatctgaaa
      361 ggctgtcttt ttgatcaggc agaccgcctc acgctcagcg gcgccatgaa gacgttcggt
      421 gccatccagc tgcacgccaa tgaagtactg gacgtcgccc ttataatcgc gcatcggctg
      481 caggtggaac acgttccaga acttcttgcc gctcttggta taattgatca gctgaacggt
      541 cacttcagtc tggttgtcaa tagcatctct gattttccga acagtggcac ggtctgtctc
      601 gggtccctgc agaaaacggc aattgcgccc caggatctct tcccgagaat attctgtcag
      661 ctgcaggaag gaatcgctcg caaaaatgat tgggttgtca ggcagtctcg gatcagtaat
      721 cacgaaattt ttctcgatcc gttccagtgt ggttgccaga aactccccac taccactacc
      781 cttgtacagc tcgtccatgc c
//
