LOCUS       sequence-103067-         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Lee YB, Cosgrave AS, Glover CP, Bienemann A, Heywood D, Hobson RJ, 
            Uney JB
  TITLE     Increased utility in the CNS of a powerful neuron-specific 
            tetracycline-regulatable adenoviral system developed using a 
            post-transcriptional enhancer.
  JOURNAL   J Gene Med. 2005 May;7(5):576-83.
  PUBMED    15580589
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Gene 
            Med."; date: "2005-05"; volume: "7(5)"; pages: "576-83"
COMMENT     Sequence Label: pXCX.CMV.tTA
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     283..302
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(398..414)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(399..415)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(504..523)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(505..523)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(568..771)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(597..617)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 aaaaccttgt tggcataaaa aggctaattg attttcgaga gtttcatact gtttttctgt
       61 aggccgtgta cctaaatgta cttttgctcc atcgcgatga cttagtaaag cacatctaaa
      121 acttttagcg ttattacgta aaaaatcttg ccagctttcc ccttctaaag ggcaaaagtg
      181 agtatggtgc ctatctaaca tctcaatggc taaggcgtcg agcaaagccc gcttattttt
      241 tacatgccaa tacaatgtag gctgctctac acctagcttc tgggcgagtt tacgggttgt
      301 taaaccttcg attccgacct cattaagcag ctctaatgcg ctgttaatca ctttactttt
      361 atctaatcta gacatatgaa ttcgatatca agcttatcga taccgtcgac ctcgagcggc
      421 cgccagtgtg atggaatctg cagaattcca gcacactggc ggccgttact agtggatccg
      481 agctcggtac caagcttggg tctccctata gtgagtcgta ttaatttcga taagccagta
      541 agcagtgggt tctctagtta gccagagagc tctgcttata tagacctccc accgtacacg
      601 cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa agtcccgttg
      661 attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg gaaatccccg
      721 tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca ccatgg
//
