LOCUS       sequence-103070-         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Streptomyces expression of codon-optimized Cas9 and custom gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Cobb RE, Wang Y, Zhao H
  TITLE     High-Efficiency Multiplex Genome Editing of Streptomyces Species 
            Using an Engineered CRISPR/Cas System.
  JOURNAL   ACS Synth Biol. 2014 Dec 8.
  PUBMED    25458909
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Dec 8."
COMMENT     Sequence Label: pCRISPomyces-2
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agctggtcga ctcgaccgac aaggcggacc tgcggctcat ctacctggcc ctcgcgcaca
       61 tgatcaagtt ccgcggccac ttcctcatcg agggcgacct gaacccggac aactccgacg
      121 tcgacaagct cttcatccag ctggtgcaga cctacaacca gctgttcgag gagaacccca
      181 tcaacgccag cggcgtcgac gccaaggcga tcctctccgc gcgcctgagc aagtcccggc
      241 gcctggagaa cctcatcgcc cagctgccgg gcgagaagaa gaacggcctc ttcggcaacc
      301 tgatcgcgct gtcgctcggc ctgaccccca acttcaagag caacttcgac ctggccgagg
      361 acgcgaagct ccagctgtcc aaggacacct acgacgacga cctggacaac ctgctcgccc
      421 agatcggcga ccagtacgcg gacctcttcc tggccgcgaa gaacctctcg gacgccatcc
      481 tgctcagcga catcctgcgg gtcaacaccg agatcaccaa ggccccgctg tcggcgagca
      541 tgatcaagcg gtacgacgag caccaccagg acctgaccct gctcaaggcc ctcgtgcgcc
      601 agcagctgcc cgagaagtac aaggagatct tcttcgacca gtccaagaac ggctacgccg
      661 gctacatcga cggcggcgcg tcgcaggagg agttctacaa gttcatcaag ccgatcctgg
      721 agaagatgga cggcaccgag gagctgctcg tcaagctgaa ccgcgaggac ctgctccgca
      781 agcagcggac cttcgacaac ggctccatcc cgcaccagat ccacctgggc gagctccacg
      841 ccatcctccg gcgccaggag gacttctacc ccttcctgaa ggacaaccgc gagaagatcg
      901 agaagatcct gaccttccgg atcccgtact acgtcggccc cctggcccgc ggcaactccc
      961 ggttcgcgtg gatgacccgg aa
//
