LOCUS       sequence-103074-         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Streptomyces expression of codon-optimized Cas9 and custom gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Cobb RE, Wang Y, Zhao H
  TITLE     High-Efficiency Multiplex Genome Editing of Streptomyces Species 
            Using an Engineered CRISPR/Cas System.
  JOURNAL   ACS Synth Biol. 2014 Dec 8.
  PUBMED    25458909
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Dec 8."
COMMENT     Sequence Label: pCRISPomyces-2
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(307..608)
                     /label=rpsL promoter
                     /note="promoter for the Xylanimonas cellulosilytica 
                     ribosomal protein S12 gene"
ORIGIN
        1 ctcgagccgg tggaagaacg agtcgtcgac cttggccatc tcgttggaga agatctcctg
       61 caggtagcag atgcggttct tgcgccgggt gtagcggcga cgggcggtcc gcttcaggcg
      121 ggtcgcctcg gcggtttcgc cgctgtcgaa gagcagggcg ccgatcaggt tcttcttgat
      181 cgagtgccgg tcggtgttgc ccaggacctt gaacttcttg gaggggacct tgtactcgtc
      241 ggtgatgacc gcccagccca cgctgttggt gccgatgtcc aggccgatgc tgtacttctt
      301 gtccattacg tctccgtcgt ctactcgagc tcacggagac cggccggccc ccgcgggtgg
      361 attcaaggct gtgcgcgccg gacagcacac ggcgcgcagc cctcacaagg ggaccgcagc
      421 cacgtaccac cggctcgatg gcccgggcct cacggcaccg ggacctgggc gtctgatgac
      481 acgccaggca cagcgagcga gcctacatga cgaaggcagg gcggtcaaaa acgaccgccc
      541 cgcctcacgt cagcagacct tgcaaggact agcggaagtc gtgtctacct gacttccgcc
      601 tgcagggcca gctcgcggac gtgctcatag tccacgacgc ccgtgatttt gtagccctgg
      661 ccgacggcca gcaggtaggc cgacaggctc atgccggccg ccgccgcctt ttcctcaatc
      721 gctcttcgtt cgtctggaag gcagtacacc ttgataggtg ggctgccctt cctggttggc
      781 ttggtttcat cagccatccg cttgccctca tctgttacgc cggcggtagc cggccagcct
      841 cgcagagcag gattcccgtt gagcaccgcc ag
//
