LOCUS       sequence-103081-         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AmpR + PLtetO-1::RBS (st7) mCherry::T1 terminator + PLlacO-1::RBS 
            (st7) gfp::Asp terminator + ColE1 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Hung M, Chang E, Hussein R, Frazier K, Shin JE, Sagawa S, Lim HN
  TITLE     Modulating the frequency and bias of stochastic switching to control
            phenotypic variation.
  JOURNAL   Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574.
  PUBMED    25087841
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-08-4"; volume: "5:4574. doi"; pages: " 
            10.1038/ncomms5574"
COMMENT     Sequence Label: pHL 1278
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      complement(229..817)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(309..328)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 caaataagct ttaatcgtac agggtagtac aaataaaaaa ggcacgtcag atgacgtgcc
       61 ttttttcttg tgagcagtga attccgttcg gctgcggcga gcggtatcag ctcactcaaa
      121 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
      181 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
      241 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
      301 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
      361 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
      421 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
      481 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
      541 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
      601 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
      661 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
      721 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
      781 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
      841 ggggtctgac gctc
//
