LOCUS       sequence-103082-         685 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AmpR + PLtetO-1::RBS (st7) gfp::T1 terminator + PLlacO-1::RBS (st7) 
            mCherry::Asp terminator + ColE1 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Hung M, Chang E, Hussein R, Frazier K, Shin JE, Sagawa S, Lim HN
  TITLE     Modulating the frequency and bias of stochastic switching to control
            phenotypic variation.
  JOURNAL   Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574.
  PUBMED    25087841
REFERENCE   2  (bases 1 to 685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-08-4"; volume: "5:4574. doi"; pages: " 
            10.1038/ncomms5574"
COMMENT     Sequence Label: pHL 1277
FEATURES             Location/Qualifiers
     source          1..685
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      34..120
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    175..191
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(364..382)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     632..651
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 aataagggcc catggtacgc gtgctagagg catcaaataa aacgaaaggc tcagtcgaaa
       61 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat
      121 ccgccgccct agacctaggc gcggatccat aaatgtgagc ggataacatt gacattgtga
      181 gcggataaca agatactccc gggtaaggag gaaaaaaaat ggtgagcaag ggcgaggagg
      241 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
      301 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
      361 ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc
      421 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
      481 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
      541 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
      601 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag acaatgggct
      661 gggaggcctc ctccgagcgg atgta
//
