LOCUS       sequence-103084-         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AmpR + fimS (off orientation)::gfp::Asp terminator + CamR + ColE1
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Hung M, Chang E, Hussein R, Frazier K, Shin JE, Sagawa S, Lim HN
  TITLE     Modulating the frequency and bias of stochastic switching to control
            phenotypic variation.
  JOURNAL   Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574.
  PUBMED    25087841
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-08-4"; volume: "5:4574. doi"; pages: " 
            10.1038/ncomms5574"
COMMENT     Sequence Label: pHL 246
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(622..650)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgctcgtttt gccggattat
       61 gggaaagaaa taatctcata aacgaaaaat taaaaagaga agaggtttga tttaacttat
      121 tgataataaa gttaaaaaaa caaataaata caagacaatt ggggccattt tgactcatag
      181 aggaaagcat cgcggacaaa ctttttcagt ttatttgttg gcttaatatt ctattgttat
      241 ctttatttat agatgtttat attgcatgag gtggtttttg gagagaagaa tgaggaagat
      301 gcgtcgagcc acagaaacgt tagctttaca tatagcggag gtgatgtgaa attaatttac
      361 aatagaaata atttacatat caaacagtta gatgcttttt gtcgtttttt aatattttta
      421 tgcttgagaa aaaatacgta acttatttat gatatggaca gtttggcccc aaatgtttca
      481 tcttttgggg gaaaactgtg cagtgttggc agtcaaactc gtttacaaaa caaagtgtac
      541 agaacgactg cccatgtcga tttagaaata gttttttgaa aggaaagcag catgcgtaaa
      601 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat
      661 gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc
      721 cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact
      781 ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt
      841 ttcaaga
//
