LOCUS       sequence-103086-         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pLtetO-1:RhyB, pCon:T7(st3) (133bps)::mCherry, pLlacO-1:sodB::gfp .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: pHL 1394
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    135..153
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    160..178
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      complement(245..272)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(364..450)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     603..620
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     primer_bind     complement(706..726)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gcttgtggat aaattgagaa cgaaagatca aaaaaaaagc cagcacccgg ctggctaagt
       61 aatactggaa gcaatgtgag caatgtcgtg ctttcaggtt ctccgcgagg gtcttcctga
      121 tcgccccggg agtatctcta tcactgatag ggatgtcaat ctctatcact gatagggagg
      181 atcctgagcg gatacatatt tgaatgtatt tagaaaaata aacaaaagag tttgtagaaa
      241 cgcaaaaagg ccatccgtca ggatggcctt ctgcttaatt tgatgcctgg cagtttatgg
      301 cgggcgtcct gcccgccacc ctccgggccg ttgcttcgca acgttcaaat ccgctcccgg
      361 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
      421 gtctttcgac tgagcctttc gttttatttg atgcctggca gttccctact ctcgcatggg
      481 gagaccccac actaccatcg gcgctacggc gtttcacttc tgagttcggc atggggtcag
      541 gtgggaccac cgcgctactg ccgccaggca aattctgttt tatcagaccg cttctgcgtt
      601 ctgatttaat ctgtatcagg ctgaaaatct tctctcatcc gccaaaacag ccaagctagc
      661 ttttatttgt agagctcatc catgccatgt gtaatcccag cagctgttac aaactcaaga
      721 aggaccatgt ggtctctctt ttcgttggga tctttcgaa
//
