LOCUS       sequence-103089-         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pLlac0-1:OxyS-mCherry, pLtetO-1:fhlA::gfp
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: pHL 100
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             120..131
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    302..318
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      complement(411..438)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(530..616)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ccctcgccct cgatctcgaa ctcgtggccg ttcacggagc cctccatgtg caccttgaag
       61 cgcatgaact ccttgatgat ggccatgtta tcctcctcgc ccttgctcac catcgtacgt
      121 ttctcctctt taataagctt tgagcctggc ttatcgccgg gcttttttat ggcaaaaaaa
      181 agcggatcct ggagatccgc aaaagttcac gttggcttta gttattcgag ttgagaaact
      241 ctcgaaacgg gcagtgactt caagggttaa aagaggtgcc gctccgtttc ccgggagtat
      301 cttgttatcc gctcacaatg tcaatgttat ccgctcacat ttatggatcc tgagcggata
      361 catatttgaa tgtatttaga aaaataaaca aaagagtttg tagaaacgca aaaaggccat
      421 ccgtcaggat ggccttctgc ttaatttgat gcctggcagt ttatggcggg cgtcctgccc
      481 gccaccctcc gggccgttgc ttcgcaacgt tcaaatccgc tcccggcgga tttgtcctac
      541 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag
      601 cctttcgttt tatttgatgc ctggcagttc cctactctcg catggggaga ccccacacta
      661 ccatcggcgc tacggcgttt cacttctgag ttcggcatgg ggtcaggtgg gaccaccgcg
      721 ctactgccgc caggcaaatt ctgttttatc agaccgcttc tgcgttctga
//
