LOCUS       sequence-103102-         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli expression of iLID (C530M).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Guntas G, Hallett RA, Zimmerman SP, Williams T, Yumerefendi H, Bear 
            JE, Kuhlman B
  TITLE     Engineering an improved light-induced dimer (iLID) for controlling 
            the localization and activity of signaling proteins.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Jan 6;112(1):112-7. doi: 
            10.1073/pnas.1417910112. Epub 2014 Dec 22.
  PUBMED    25535392
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1417910112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-01-6- 6"; volume: "112"; issue: "1"; pages: "112-7"
COMMENT     Sequence Label: pQE-80L iLID (C530M)
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    43..59
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             78..89
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             108..125
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             141..578
                     /codon_start=1
                     /product="improved light-inducible dimer when used with 
                     SspB nano or SspB micro (Guntas et al., 2015)"
                     /label=iLID
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNVFHLQPMRDYKGDVQ
                     YFIGVQLDGTERLHGAAEREAVMLIKKTAFQIAEAANDENYF"
     terminator      603..697
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 tttatttgct ttgtgagcgg ataacaatta taatagattc aattgtgagc ggataacaat
       61 ttcacacaga attcattaaa gaggagaaat taactatgag aggatcgcat caccatcacc
      121 atcacggatc cggggagttt ctggcaacca cactggaacg gatcgagaaa aatttcgtga
      181 ttactgatcc gagactgcct gacaacccaa tcatttttgc gagcgattcc ttcctgcagc
      241 tgacagaata ttctcgggaa gagatcctgg ggcgcaattg ccgttttctg cagggacccg
      301 agacagaccg tgccactgtt cggaaaatca gagatgctat tgacaaccag actgaagtga
      361 ccgttcagct gatcaattat accaagagcg gcaagaagtt ctggaacgtg ttccacctgc
      421 agccgatgcg cgattataag ggcgacgtcc agtacttcat tggcgtgcag ctggatggca
      481 ccgaacgtct tcatggcgcc gctgagcgtg aggcggtcat gctgatcaaa aagacagcct
      541 ttcagattgc tgaggcagcg aacgacgaaa attactttta aaagcttaat tagctgagct
      601 tggactcctg ttgatagatc cagtaatgac ctcagaactc catctggatt tgttcagaac
      661 gctcggttgc cgccgggcgt tttttattgg tgagaatcca agctagcttg gcgagatttt
      721 caggagctaa ggaagctaaa atggag
//
