LOCUS       sequence-103105-         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli expression of iLID (C530M).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Guntas G, Hallett RA, Zimmerman SP, Williams T, Yumerefendi H, Bear 
            JE, Kuhlman B
  TITLE     Engineering an improved light-induced dimer (iLID) for controlling 
            the localization and activity of signaling proteins.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Jan 6;112(1):112-7. doi: 
            10.1073/pnas.1417910112. Epub 2014 Dec 22.
  PUBMED    25535392
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1417910112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-01-6- 6"; volume: "112"; issue: "1"; pages: "112-7"
COMMENT     Sequence Label: pQE-80L iLID (C530M)
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(45..482)
                     /codon_start=1
                     /product="improved light-inducible dimer when used with 
                     SspB nano or SspB micro (Guntas et al., 2015)"
                     /label=iLID
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNVFHLQPMRDYKGDVQ
                     YFIGVQLDGTERLHGAAEREAVMLIKKTAFQIAEAANDENYF"
     CDS             complement(498..515)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             534..545
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    564..580
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(588..594,595..611,612..617,618..632))
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    596..612
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(656..674)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tggatctatc aacaggagtc caagctcagc taattaagct tttaaaagta attttcgtcg
       61 ttcgctgcct cagcaatctg aaaggctgtc tttttgatca gcatgaccgc ctcacgctca
      121 gcggcgccat gaagacgttc ggtgccatcc agctgcacgc caatgaagta ctggacgtcg
      181 cccttataat cgcgcatcgg ctgcaggtgg aacacgttcc agaacttctt gccgctcttg
      241 gtataattga tcagctgaac ggtcacttca gtctggttgt caatagcatc tctgattttc
      301 cgaacagtgg cacggtctgt ctcgggtccc tgcagaaaac ggcaattgcg ccccaggatc
      361 tcttcccgag aatattctgt cagctgcagg aaggaatcgc tcgcaaaaat gattgggttg
      421 tcaggcagtc tcggatcagt aatcacgaaa tttttctcga tccgttccag tgtggttgcc
      481 agaaactccc cggatccgtg atggtgatgg tgatgcgatc ctctcatagt taatttctcc
      541 tctttaatga attctgtgtg aaattgttat ccgctcacaa ttgaatctat tataattgtt
      601 atccgctcac aaagcaaata aattttttat gatttctcga ggtgaagacg aaagggcctc
      661 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
      721 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattc
//
