LOCUS       sequence-103114-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET-based biosensor for monitoring PKA activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Depry C, Allen MD, Zhang J
  TITLE     Visualization of PKA activity in plasma membrane microdomains.
  JOURNAL   Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10.1039/c0mb00079e. Epub 2010 
            Sep 14.
  PUBMED    20838685
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/c0mb00079e"; journalName: "Mol Biosyst"; date: "2011-01"; 
            volume: "7"; issue: "1"; pages: "52-8"
COMMENT     Sequence Label: pcDNA3-AKAR4
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     454..475
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(581..602)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(842..861)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 taagcatttc caaatactac taggagaaga cggtaacctt ttattgaatg acatttccac
       61 taatgggacc tggttaaatg ggcaaaaagt cgagaagaac agcaatcagt tactgtctca
      121 aggtgatgaa ataaccgttg gtgtaggcgt ggaatcagat attttatctc tggtcatttt
      181 cataaacgac aaatttaagc agtgcctcga gcagaacaaa gttgatcgct ctgcaggtaa
      241 gccaggcagc ggcgagggca gcaccaaggg cctgcgtcgc gccaccctgg tggacggcgg
      301 caccggcggc agcgagctca tgggcggcgt gcagctcgcc gaccactacc agcagaacac
      361 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccaa
      421 gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
      481 cgccgggatc actctcggca tggacgagct gtacaaggga ggtaccggtg gatctatggt
      541 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
      601 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
      661 gctgaccctg aagctgatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
      721 gaccaccctg ggctacggcc ttcagtgctt cgcccgctac cccgaccaca tgaagcagca
      781 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
      841 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
      901 ccgcatcgag ctgaa
//
