LOCUS       sequence-103127-         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of proteins fused to an N-terminal monomeric 
            streptavidin tag (mSA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Beck DB, Narendra V, Drury WJ 3rd, Casey R, Jansen PW, Yuan ZF, 
            Garcia BA, Vermeulen M, Bonasio R
  TITLE     In Vivo Proximity Labeling for the Detection of Protein-Protein and 
            Protein-RNA Interactions.
  JOURNAL   J Proteome Res. 2014 Dec 5;13(12):6135-43. doi: 10.1021/pr500196b. 
            Epub 2014 Oct 29.
  PUBMED    25311790
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/pr500196b";
            journalName: "J Proteome Res"; date: "2014-12-5- 5"; volume: "13"; 
            issue: "12"; pages: "6135-43"
COMMENT     Sequence Label: pINTO-N-SA
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..36
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    39..57
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     67..91
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      179..188
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(782..799)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    788..899
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctatataagc agagctctcc ctatcagtga tagagatctc cctatcagtg atagagatcg
       61 tcgacgagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
      121 cctccataga agacaccggg accgatccag cctccggact ctagcgttta aacttaaggc
      181 caccatgggc gaccccagca aagattctaa agcccaggtg tctgccgccg aagccggaat
      241 tacaggcaca tggtataatc agctgggatc tacatttatt gtgacagccg gcgccgatgg
      301 agccctgaca ggaacatatg aatctgccgt gggaaatgcc gaatccagat acaccctgac
      361 aggaagatat gattctgccc ctgccacaga tggatctgga acagccctgg gatggagagt
      421 ggcctggaag aataattata gaaacgccca tagcgccaca acatggtctg gccagtatgt
      481 gggaggcgcc gaagccagaa ttaacacaca gtggaccctg acatctggaa caacagaagc
      541 caatgcctgg aaaagcactc tgagaggaca tgatacattt acaaaagtga aacctagcgc
      601 cgcctctatc gatgccgcca agaaggccgg agtgaacaat ggcaatcctc tggatgccgt
      661 gcagcagacc ggtagcggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc
      721 agatatccag cacagtggcg gccgctcgag tctagagggc ccgtttaaac ccgctgatca
      781 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
      841 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
      901 cattgtctga gtaggtgtca ttctattctg gggg
//
