LOCUS       sequence-103131-         642 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Wild-type OmpA with aromatic girdle residues replaced by leucine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Stapleton JA, Whitehead TA, Nanda V
  TITLE     Computational redesign of the lipid-facing surface of the outer 
            membrane protein OmpA.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
  PUBMED    26199411
REFERENCE   2  (bases 1 to 642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2015 Jul 21. pii: 201501836."
COMMENT     Sequence Label: pJexpress414_OmpA171_WTnoGirdles
FEATURES             Location/Qualifiers
     source          1..642
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..63
                     /codon_start=1
                     /product="signal peptide from the E. coli outer membrane 
                     protein OmpA"
                     /label=OmpA signal peptide
                     /translation="MKKTAIAIAVALAGFATVAQA"
     CDS             523..549
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             604..621
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     CDS             622..639
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgaaaaaga ccgctatcgc gattgcagtg gcactggctg gtttcgctac cgtagcgcag
       61 gccgcgccga acgacaacac cttgtatact ggtgctaaac tgggcttgtc ccagtaccat
      121 gatactggtt tcatcaataa taatggcccg acccatgaaa accaactggg cgctggtgct
      181 tttggtggtt tgcaggttaa cccgtatgtt ggcttggaaa tgggtttgga cttgttaggt
      241 cgtatgccgt ataaaggcag cgttgaaaac ggtgcatata aagctcaggg cgttcaactg
      301 accgctaaac tgggtttgcc aatcactgac gacctggata tctatactcg tctgggtggc
      361 atggtatggc gtgcagatac taaatccaac gtttatggta aaaaccacga taccggcgtt
      421 tctccggtct tggctggcgg tgttgagttg gcgatcactc ctgaaatcgc tacccgtctg
      481 gaattgcagt tgaccaataa tatcggtgac gcgcatacca tctacccata cgatgttcca
      541 gattacgctg gcactcgtcc ggataacggc atgctgagcc tgggtgtttc ctaccgtttc
      601 ggttgttgcc ctggttgctg tcaccatcac caccatcact aa
//
