LOCUS       sequence-103132-         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Krajcikova D, Hartley RW
  TITLE     A new member of the bacterial ribonuclease inhibitor family from 
            Saccharopolyspora erythraea.
  JOURNAL   FEBS Lett 2004 Jan 16;557(1-3):164-8.
  PUBMED    14741361
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett";
            date: "2004-01-16"; volume: "557(1-3)"; pages: "164-8"
COMMENT     Sequence Label: pStI6
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    72..96
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             111..133
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             530..547
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(600..618)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      614..661
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga
       61 ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac
      121 tttaagaagg agatatacca tgggcgggtt ggaggaaccc tccgctgacg tcagcgccgt
      181 caaagccgcc gaggacgccg agcgacgcgg cgcggcggcg cacgtcctgg acggttcgga
      241 gctgctcagc aagcgcgcgg cgctcgacgg catcgccgcg gtcctggact tcccggagtg
      301 ggcgggccgc aacctcgacg ccctctacga ctgcctcacc gatctgagct ggctgcccga
      361 gggcgagcac gtgctgatct ggtccggcta ccaggcactg gccgactacg acccgaaggc
      421 ctaccggaag atcagcgcgg tgctgaagga ggcgtcggag accagctttt gcggccggac
      481 gtttaccgcg gtcctgacgc gcaactgaaa gcttgcggcc gcactcgagc accaccacca
      541 ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
      601 cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
      661 gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta
      721 agcgcggcgg gtgtggtggt tacgcgcaac gtgaccgcta cacttgccag cgccctagcg
      781 cccgctcctt ttcgctttct t
//
