LOCUS       sequence-103133-         481 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV vector with CaMKIIa promoter, EGFP, and W3SL(shorten expression 
            cassette with relatively high expression to maximize expression of 
            larger genes) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Choi JH, Yu NK, Baek GC, Bakes J, Seo D, Nam HJ, Baek SH, Lim CS, 
            Lee YS, Kaang BK
  TITLE     Optimization of AAV expression cassettes to improve packaging 
            capacity and transgene expression in neurons.
  JOURNAL   Mol Brain. 2014 Mar 11;7:17. doi: 10.1186/1756-6606-7-17.
  PUBMED    24618276
REFERENCE   2  (bases 1 to 481)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Brain."; date: "2014-03-11"; volume: "7:17. doi"; pages: " 
            10.1186/1756-6606-7-17"
COMMENT     Sequence Label: pAAV-CW3SL-EGFP
FEATURES             Location/Qualifiers
     source          1..481
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      465..474
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gagcgcgcag agagggagtg gccaactcca tcactagggg ttcctgcggc cgcagatgct
       61 gcacacttgt ggactaagtt tgttcgcatc cccttctcca accccctcag tacatcaccc
      121 tgggggaaca gggtccactt gctcctgggc ccacacagtc ctgcagtatt gtgtatataa
      181 ggccagggca aagaggagca ggttttaaag tgaaaggcag gcaggtgttg gggaggcagt
      241 taccggggca acgggaacag ggcgtttcgg aggtggttgc catggggacc tggatgctga
      301 cgaaggctcg cgaggctgtg agcagccaca gtgccctgct cagaagcccc aagctcgtca
      361 gtcaagccgg ttctccgttt gcactcagga gcacgggcag gcgagtggcc cctagttctg
      421 ggggcagcgt gaaaagacgg tatcgataag cttgattcga gctagccacc atggtgagca
      481 a
//
