LOCUS       sequence-103136-         514 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV vector with CaMKIIa promoter, EGFP, and W3SL(shorten expression 
            cassette with relatively high expression to maximize expression of 
            larger genes) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Choi JH, Yu NK, Baek GC, Bakes J, Seo D, Nam HJ, Baek SH, Lim CS, 
            Lee YS, Kaang BK
  TITLE     Optimization of AAV expression cassettes to improve packaging 
            capacity and transgene expression in neurons.
  JOURNAL   Mol Brain. 2014 Mar 11;7:17. doi: 10.1186/1756-6606-7-17.
  PUBMED    24618276
REFERENCE   2  (bases 1 to 514)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Brain."; date: "2014-03-11"; volume: "7:17. doi"; pages: " 
            10.1186/1756-6606-7-17"
COMMENT     Sequence Label: pAAV-CW3SL-EGFP
FEATURES             Location/Qualifiers
     source          1..514
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(90..110)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 acgagctgta caagtaaatc gaattccgct cgagataatc aacctctgga ttacaaaatt
       61 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
      121 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
      181 tataaatcct ggttagttct tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
      241 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgtttatttg tgaaatttgt
      301 gatgctattg ctttatttgt aaccatctag ctttatttgt gaaatttgtg atgctattgc
      361 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
      421 tatgtttcag gttcaggggg agatgtggga ggttttttaa agcggccgca ggaaccccta
      481 gtgatggagt tggccactcc ctctctgcgc gctc
//
