LOCUS       sequence-103138-         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV vector with CaMKIIa promoter, EGFP, WPRE, and bGHpA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Choi JH, Yu NK, Baek GC, Bakes J, Seo D, Nam HJ, Baek SH, Lim CS, 
            Lee YS, Kaang BK
  TITLE     Optimization of AAV expression cassettes to improve packaging 
            capacity and transgene expression in neurons.
  JOURNAL   Mol Brain. 2014 Mar 11;7:17. doi: 10.1186/1756-6606-7-17.
  PUBMED    24618276
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Brain."; date: "2014-03-11"; volume: "7:17. doi"; pages: " 
            10.1186/1756-6606-7-17"
COMMENT     Sequence Label: pAAV-CWB-EGFP
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    40..628
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(93..113)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(511..522)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    641..752
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 acgagctgta caagtaaatc gaattcctgc agccccgata atcaacctct ggattacaaa
       61 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
      121 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
      181 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
      241 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
      301 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
      361 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
      421 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
      481 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
      541 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
      601 cggatctccc tttgggccgc ctccccgcat cggggggatc ctgtgccttc tagttgccag
      661 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
      721 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
      781 ct
//
