LOCUS       sequence-103162-         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Mouse p53 (residues 1-87) in e. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Daughdrill GW, Borcherds WM, Wu H
  TITLE     Disorder predictors also predict backbone dynamics for a family of 
            disordered proteins.
  JOURNAL   PLoS One. 2011;6(12):e29207. doi: 10.1371/journal.pone.0029207. Epub
            2011 Dec 15.
  PUBMED    22195023
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12):e29207. doi"; pages: " 
            10.1371/journal.pone.0029207. Epub 2011 Dec 15"
COMMENT     Sequence Label: pET28-p53 (Mouse 1-87)
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(18..35)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(312..329)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(339..356)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             375..397
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    412..436
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(436..455)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(437..455)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(539..558)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ttagcagccg gatctcagtg gtggtggtgg tggtgctcga gttagggggt ggngggggcg
       61 ggggcgacgg gacctggggt ttcggtgact ggatcttgtg cagcaggggc gccagagaca
      121 cgtaaggctt cagagggacc ttcaaaaaat tcttcaacat cttggggtaa taataaatcg
      181 tccatacagt gagggctagg taaaatatct tcgggaggca gtaatttcca taaaccagag
      241 aatgtttctt gtgataaagg cagttcaagg gaaatatcgg attggctttc ttccattgcg
      301 gtcatcatat ggctgccgcg cggcaccagg ccgctgctgt gatgatgatg atgatggctg
      361 ctgcccatgg tatatctcct tcttaaagtt aaacaaaatt atttctagag gggaattgtt
      421 atccgctcac aattccccta tagtgagtcg tattaatttc gcgggatcga gatctcgatc
      481 ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc
      541 tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct
      601 tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc
      661 ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc
      721 ttcctaatgc aggagtcgca taagggagag cgtcgagatc ccggacacca tcgaatggcg
      781 c
//
