LOCUS       sequence-103164-        1008 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast integration plasmid targeted to the HO loci with heterodimeric
            Hsp82. Digest with NotI prior to addition to yeast..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Mishra P, Bolon DN
  TITLE     Designed Hsp90 heterodimers reveal an asymmetric ATPase-driven 
            mechanism in vivo.
  JOURNAL   Mol Cell. 2014 Jan 23;53(2):344-50. doi: 
            10.1016/j.molcel.2013.12.024.
  PUBMED    24462207
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2013.12"; journalName: "Mol Cell"; date: 
            "2014-01-23- 23"; volume: "53"; issue: "2"; pages: "344-50"
COMMENT     Sequence Label: pHOHO2_Hsp82viiA
FEATURES             Location/Qualifiers
     source          1..1008
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             82..99
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttacaacttt ttttacttct tgctcattag aaagaaagca tagcaatcta atctaagttt
       61 tctagaacta gtatgggtgg tcaccaccat caccaccacg gtggtcatat ggctagtgaa
      121 acttttgaat ttcaagctga aattactcag ttgatgagtt tgatcatcaa caccgtctat
      181 tctaacaagg aaattttctt gagagaactg atatctaatg cctcggatgc gttggataaa
      241 attagataca aatctttgtc tgatccaaag caattggaaa cagaaccaga tctctttatt
      301 agaatcactc caaagccaga gcaaaaagtt ttggaaatca gagattctgg tattggtatg
      361 accaaggctg aattgattaa taacttgggt accattgcca agtctggtac caaagccttc
      421 atggaagctc tatctgctgg tgccgatgta tccatgattg gtcaattcgg tgttggtttt
      481 tactctttat tcttagttgc cgacagagtt caggttattt caaagagcaa cgacgacgaa
      541 caatacatct gggaatccaa cgctggtggt tctttcactg ttactctaga cgaagttaat
      601 gaaagaattg gtaggggtac catcttgagg ttattcttga aagatgacca attggagtac
      661 ttggaagaaa agagaataaa ggaagttatc aagagacatt ctgagttcgt ggcctaccca
      721 atccaattag tcgtcaccaa ggaagttgaa aaggaagttc caattccaga agaagaaaag
      781 aaagacgagg aaaagaagga tgaggaaaag aaggatgaag acgacaagaa accaaaactc
      841 gaggaagtcg atgaagaaga ggaaaagaag ccaaagacga aaaaagttaa agaagaagtt
      901 caagagatag aagaactaaa caagactaag cctttgtgga ctagaaaccc atctgatatc
      961 actcaagaag aatacaatgc tttctataag tctatttcaa acgactgg
//
