LOCUS       sequence-103165-         688 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian cell expression plasmid; drives constitutive, low-level 
            expression from retroviral LTR; retroviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Kofoed EM, Vance RE
  TITLE     Innate immune recognition of bacterial ligands by NAIPs determines 
            inflammasome specificity.
  JOURNAL   Nature. 2011 Aug 28;477(7366):592-5. doi: 10.1038/nature10394.
  PUBMED    21874021
REFERENCE   2  (bases 1 to 688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-08-28- 28"; volume: "477"; issue: "7366"; 
            pages: "592-5"
COMMENT     Sequence Label: mscv2.2
FEATURES             Location/Qualifiers
     source          1..688
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    92..658
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    108..660
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(252..269)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     479..498
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 tcactccttc tctaggcgcc ggaattagat ctctcgagat cgatgcggcc gcgtttaaac
       61 gtcgacggta tcgataagct tgatatcgaa ttccgccccc ccccctaacg ttactggccg
      121 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
      181 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
      241 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
      301 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
      361 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
      421 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
      481 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
      541 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa
      601 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata
      661 tggccacaac catggtgagc aagggcga
//
