LOCUS       sequence-103167-         688 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  6myc-tagged PrgJ (SPI-1 rod protein) from Salmonella typhimurium, 
            under the constitutive, moderate-level retroviral LTR promoter for 
            mammalian cell expression; IRES-GFP reporter downstream of PrgJ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Kofoed EM, Vance RE
  TITLE     Innate immune recognition of bacterial ligands by NAIPs determines 
            inflammasome specificity.
  JOURNAL   Nature. 2011 Aug 28;477(7366):592-5. doi: 10.1038/nature10394.
  PUBMED    21874021
REFERENCE   2  (bases 1 to 688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-08-28- 28"; volume: "477"; issue: "7366"; 
            pages: "592-5"
COMMENT     Sequence Label: mscv2.2-6mycPrgJ
FEATURES             Location/Qualifiers
     source          1..688
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             50..79
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             89..118
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             128..157
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             167..196
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             206..235
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             269..298
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tcactccttc tctaggcgcc ggaattagat ctatcgattt aaagctatgg agcaaaagct
       61 catttctgaa gaggacttga atgaaatgga gcaaaagctc atttctgaag aggacttgaa
      121 tgaaatggag caaaagctca tttctgaaga ggacttgaat gaaatggagc aaaagctcat
      181 ttctgaagag gacttgaatg aaatggagca aaagctcatt tctgaagagg acttgaatga
      241 aatggagagc ttgggcgacc tcaccatgga gcaaaagctc atttctgaag aggacttgaa
      301 ttcaggccgg ccagcggccg catcgattgc aactattgtc cctgagaatg ccgttatagg
      361 gcaggcggtc aatatcaggt ctatggaaac ggacattgtc tcgctggatg accggctact
      421 ccaggctttt tctggttcgg cgattgccac ggctgtggat aaacagacga ttaccaacag
      481 gattgaggac cctaatctgg tgacggatcc taaagagctg gctatttcgc aagagatgat
      541 ttcagattat aacctgtatg tttctatggt cagtaccctt actcgtaaag gagtcggggc
      601 tgttgaaacg ctattacgct catgagtcga cggtatcgat aagcttgata tcgaattccg
      661 cccccccccc taacgttact ggccgaag
//
