LOCUS       sequence-103171-         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PCR template for en passant (two-step Red-mediated) mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Tischer BK, von Einem J, Kaufer B, Osterrieder N
  TITLE     Two-step red-mediated recombination for versatile high-efficiency 
            markerless DNA manipulation in Escherichia coli.
  JOURNAL   Biotechniques. 2006 Feb;40(2):191-7.
  PUBMED    16526409
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques."; date: "2006-02"; volume: "40(2)"; pages: "191-7"
COMMENT     Sequence Label: pEPkan-S2
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    88..312
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     301..318
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        338..356
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     338..355
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtggggatac cccctagagc
       61 cccagctggt tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc
      121 tattgtcttc ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat
      181 gacacctact cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag
      241 tggcaccttc cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac
      301 tagaaggcac agtcgaggct gatcagcgag ctctagcatt taggtgacac tatagaatag
      361 ggccctctag atgcatgctc gagcggccgc cagtgtgatg gatatctagg gataacaggg
      421 taatgccagt gttacaacca attaaccaat tctgattaga aaaactcatc gagcatcaaa
      481 tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc
      541 tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg
      601 tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata
      661 aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagc
      721 ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca
      781 ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagac
//
