LOCUS       sequence-103172-         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast integration plasmid targeted to the HO loci with heterodimeric
            Hsp82. Digest with NotI prior to addition to yeast..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Mishra P, Bolon DN
  TITLE     Designed Hsp90 heterodimers reveal an asymmetric ATPase-driven 
            mechanism in vivo.
  JOURNAL   Mol Cell. 2014 Jan 23;53(2):344-50. doi: 
            10.1016/j.molcel.2013.12.024.
  PUBMED    24462207
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2013.12"; journalName: "Mol Cell"; date: 
            "2014-01-23- 23"; volume: "53"; issue: "2"; pages: "344-50"
COMMENT     Sequence Label: pHOHO2_Hsp82viiA
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    92..113
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
       61 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
      121 cccaggcttt acactttatg cttcctcgag atccgatatc gccgtggcgg ccgccagctg
      181 aagcttaatt atcctgggca cgagtgaaac aaagctaaaa cctttattta gcatggccat
      241 tgaatgtaac aattatatat atcgcaagca caaaaaatca aggagagaga actaccactt
      301 tgttcatgtg tacaatgttc attatctcca taagcaaaaa aaaaaaaata gaaaacatat
      361 gctataaggt tgatattctc acgagtaagc ggcacttgct acttattgac attgcagatt
      421 tttggctaca gaaatagtat attagagatt ataattgcta atcaaatcaa aatataaaat
      481 tagtaaacca aaccatttat acccttcctt agtagttatg gattgttttt taatgatatt
      541 tctgcaaacc aaagaaagat tgttatccag atagaattta gttttgatat tcattttttt
      601 gttgaagatt gaacgccata tctgggcctc ataattcaaa agacggtgcc attatcggta
      661 gcgtttcgca ttgtactgga tttcagaaat ttcacagttg atgaatcgaa aagaatggtc
      721 tcattgcaac acgtaaggtt aagatgtccc tttttaccat tataggcaat aaatgaatca
      781 taaaacgacc gtatactggt gaaatagtag ggagaacgag tacctgtagt aaaaagtata
      841 aatcatagtt aatcgggcaa tgtccctcga tcaaggagta ttgtgtcatg ttcgagacaa
      901 acgccaacat ttttgtttct tttggacaaa tgttgtttgc atttatgatc cgttatattt
      961 tga
//
