LOCUS       sequence-103173-        1024 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast integration plasmid targeted to the HO loci with heterodimeric
            Hsp82. Digest with NotI prior to addition to yeast..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Mishra P, Bolon DN
  TITLE     Designed Hsp90 heterodimers reveal an asymmetric ATPase-driven 
            mechanism in vivo.
  JOURNAL   Mol Cell. 2014 Jan 23;53(2):344-50. doi: 
            10.1016/j.molcel.2013.12.024.
  PUBMED    24462207
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2013.12"; journalName: "Mol Cell"; date: 
            "2014-01-23- 23"; volume: "53"; issue: "2"; pages: "344-50"
COMMENT     Sequence Label: pHOHO2_Hsp82viiA
FEATURES             Location/Qualifiers
     source          1..1024
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(206..393)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
ORIGIN
        1 gagcatgtat catatggtcc agaaacccta tacctgtgtg gacgttaatc acttgcgatt
       61 gtgtggcctg ttctgctact gcttctgcct ctttttctgg gaagatcgag tgctctatcg
      121 ctaggggacc accctttaaa gagatcgcaa tctgaatctt ggtttcattt gtaatacgct
      181 ttactagggc tttctgctct gtcatctttg ccttcgttta tcttgcctgc tcatttttta
      241 gtatattctt cgaagaaatc acattacttt atataatgta taattcatta tgtgataatg
      301 ccactcgcta agaaaaaaaa agagtcatcc gctaggtgga aaaaaaaaaa tgaaaatcat
      361 taccgaggca taaaaaaata tagagtgtac tagaggaggc caagagtaat agaaaaagaa
      421 aattgcggga aaggactgtg ttatgacttc cctgactaat gccgtgttca aacgatacct
      481 ggcagtgact cctagcgctc accaagctct taaaacggga atttatggtg cactctggcg
      541 cgccctgggg gaacaacttc acagaatgtt ttgtcatatt gtcgaagtgg tcacaaaaca
      601 agagaagttc cgccaattat aaaaagggaa cccgtatatt tcagcttcac ggatgatttc
      661 cagggtgaga gtactgtata tgggcttacg atagaaggcc ataaaaattt cttgcttggc
      721 aacaaaatag aagtgaaatc atgtcgaggc tgctgtgtgg gagaacagca taaaatatca
      781 caaaaaaaga atctaaaaca ctgtgttgct tgtcccagaa agggaatcaa gtatttttat
      841 aaagattgga gtggtaaaaa tcgagtatgt gctagatgct atggaagata caaattcagc
      901 ggtcatcact gtataaattg caagtatgta ccagaagcac gtgaagtgaa aaaggcaaaa
      961 gacaaaggcg aaaaattggg cattacgccc gaaggtttgc cagttaaagg accagagtgt
     1021 ataa
//
