LOCUS       sequence-103179-         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a ligation-independent cloning (LIC) retroviral vector for stable 
            gene transduction in mammalian cells. Neo selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Patel A, Munoz A, Halvorsen K, Rai P
  TITLE     Creation and validation of a ligation-independent cloning (LIC) 
            retroviral vector for stable gene transduction in mammalian cells.
  JOURNAL   BMC Biotechnol. 2012 Jan 16;12:3. doi: 10.1186/1472-6750-12-3.
  PUBMED    22248071
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2012-01-16"; volume: "12:3. doi"; pages: " 
            10.1186/1472-6750-12-3"
COMMENT     Sequence Label: pBLIC-neo
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     197..217
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(265..681)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(281..297)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(313..335)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcccg gactatggtt
       61 gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt
      121 ccacacctgg ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag
      181 cctggggact ttccacaccc taactgacac acattccaca gggtcgagct cacattccac
      241 gtgagatggt gtgcggatcc ggccggcgcc tagagaagga gtgagggctg gataaaggga
      301 ggatcgaggc ggggtcgaac gaggaggttc aagggggaga gacggggcgg atggaggaag
      361 aggaggcgga ggcttagggt gtacaaaggg cttgacccag ggaggggggt caaaagccaa
      421 ggcttcccag gtcacgatgt aggggacctg gtctgggtgt ccatgcgggc caggtgaaaa
      481 gaccttgatc ttaacctggg tgatgaggtc tcggttaaag gtgccgtctc gcggccatcc
      541 gacgttaaag gttggccatt ctgcagagca gaaggtaacc caacgtctct tcttgacatc
      601 taccgactgg ttgtgagcga tccgctcgac atctttccag tgacctaagg tcaaacttaa
      661 gggagtggta acagtctggc cctaattttc agacaaatac agaaacacag tcagacagag
      721 acaacacaga acgatgctgc agcgctgcag cagacaagac gcgcggcttc gg
//
