LOCUS       sequence-103183-         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a ligation-independent cloning (LIC) retroviral vector for stable 
            gene transduction in mammalian cells. Puro selection. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Patel A, Munoz A, Halvorsen K, Rai P
  TITLE     Creation and validation of a ligation-independent cloning (LIC) 
            retroviral vector for stable gene transduction in mammalian cells.
  JOURNAL   BMC Biotechnol. 2012 Jan 16;12:3. doi: 10.1186/1472-6750-12-3.
  PUBMED    22248071
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2012-01-16"; volume: "12:3. doi"; pages: " 
            10.1186/1472-6750-12-3"
COMMENT     Sequence Label: pBLIC-puro
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     196..216
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(264..680)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(280..296)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(312..334)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcgtgg actatggttg
       61 ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc
      121 cacacctggt tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc
      181 ctggggactt tccacaccct aactgacaca cattccacag ggtcgagctc acattccacg
      241 tgagatggtg tgcggatccg gccggcgcct agagaaggag tgagggctgg ataaagggag
      301 gatcgaggcg gggtcgaacg aggaggttca agggggagag acggggcgga tggaggaaga
      361 ggaggcggag gcttagggtg tacaaagggc ttgacccagg gaggggggtc aaaagccaag
      421 gcttcccagg tcacgatgta ggggacctgg tctgggtgtc catgcgggcc aggtgaaaag
      481 accttgatct taacctgggt gatgaggtct cggttaaagg tgccgtctcg cggccatccg
      541 acgttaaagg ttggccattc tgcagagcag aaggtaaccc aacgtctctt cttgacatct
      601 accgactggt tgtgagcgat ccgctcgaca tctttccagt gacctaaggt caaacttaag
      661 ggagtggtaa cagtctggcc ctaattttca gacaaataca gaaacacagt cagacagaga
      721 caacacagaa cgatgctgca gcgctgcagc agacagacgc gcggcttcgg ttccaaaccg
      781 aagcaaaaat tcagac
//
