LOCUS       sequence-103189-         668 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Guinea Pig p53 (residues 1-88) in e. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Daughdrill GW, Borcherds WM, Wu H
  TITLE     Disorder predictors also predict backbone dynamics for a family of 
            disordered proteins.
  JOURNAL   PLoS One. 2011;6(12):e29207. doi: 10.1371/journal.pone.0029207. Epub
            2011 Dec 15.
  PUBMED    22195023
REFERENCE   2  (bases 1 to 668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12):e29207. doi"; pages: " 
            10.1371/journal.pone.0029207. Epub 2011 Dec 15"
COMMENT     Sequence Label: pET28-p53 (Guinea Pig 1-88)
FEATURES             Location/Qualifiers
     source          1..668
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             367..384
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(437..455)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      451..498
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(622..641)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggtgccgcg cggcagccat atgatggaag aaccacactc cgatcttagt
      121 attgaacccc ccttatccca agaaacattt agcgatttat ggaaattact gcccgaaaat
      181 aacgttttat ccgattccct ttcccctcca atggaccacc tcctgttatc tcctgaagaa
      241 gttgcctcct ggttaggtga aaatcctgat ggcgatggac atgttagcgc cgcccccgta
      301 tctgaagccc ctacctctgc tggacccgcc cttgttgccc ccgcacccgc cacctcttaa
      361 ctcgagcacc accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct
      421 gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
      481 gtcttgaggg gttttttgct gaaaggagga actatatccg gattggcgaa tgggacgcgc
      541 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
      601 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
      661 ccggcttt
//
