LOCUS       sequence-103190-         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Kinet JP, Metzger H, Hakimi J, Kochan J
  TITLE     A cDNA presumptively coding for the alpha subunit of the receptor 
            with high affinity for immunoglobulin E.
  JOURNAL   Biochemistry 1987 Jul 28;26(15):4605-10.
  PUBMED    2959318
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry"; date: "1987-07-28"; volume: "26(15)"; pages: 
            "4605-10"
COMMENT     Sequence Label: pGEM-rat FceRI alpha
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    80..101
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(116..121,122..139,140..146)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    154..170
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     159..181
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     178..194
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     178..194
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        212..230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     212..229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    237..293
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
ORIGIN
        1 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
       61 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
      121 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
      181 gaaacagcta tgaccatgat tacgccaagc tatttaggtg acactataga atactcaagc
      241 ttgcatgcct gcaggtcgac tctagaggat ccccgggtac cgagctcgaa ttccgcctac
      301 ttttgaagcc gtagctcact ggtgcagtta gcacctgaag gcacaggggc aatggatact
      361 ggaggatctg cccggctgtg cctagcatta gtgctcatat ctctgggtgt catgctaaca
      421 gccactcaga aatctgtagt gtccttggac ccaccgtgga ttagaatact tacaggagat
      481 aaagtgactc ttatatgcaa tgggaacaat tcctctcaaa tgaactctac taaatggatc
      541 cacaatgata gcatctctaa tgtgaaatcg tcacattggg tcattgtgag tgccaccatt
      601 caagacagtg gaaaatacat atgtcagaag caaggatttt ataagagcaa acctgtgtac
      661 ttgaacgtga tgcaagagtg gctgctgctc caatcttctg ctgacgtggt cttagacaat
      721 ggatcctttg acatcagatg ccgtagctgg aagaaatgga aagtccacaa ggtgatctac
      781 tacaaggacg acattgcttt caagtactct tatgacagca acaacatctc cattagaaag
      841 gccacattta atgacagtgg cagctaccac tgcacaggct atttgaacaa ggttgaatgt
      901 aaatctgata aattcagtat tgctgtagta aaagattaca caattgagta tcgttggcta
      961 caactcattt tcccatcatt ggcggtgat
//
