LOCUS       sequence-103193-         725 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Guinea Pig p53 (residues 1-88) in e. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Daughdrill GW, Borcherds WM, Wu H
  TITLE     Disorder predictors also predict backbone dynamics for a family of 
            disordered proteins.
  JOURNAL   PLoS One. 2011;6(12):e29207. doi: 10.1371/journal.pone.0029207. Epub
            2011 Dec 15.
  PUBMED    22195023
REFERENCE   2  (bases 1 to 725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12):e29207. doi"; pages: " 
            10.1371/journal.pone.0029207. Epub 2011 Dec 15"
COMMENT     Sequence Label: pET28-p53 (Guinea Pig 1-88)
FEATURES             Location/Qualifiers
     source          1..725
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(320..337)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(347..364)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             383..405
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    420..444
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(444..463)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(445..463)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(547..566)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cttgttagca gccggatctc agctggtggt ggtggtggtg ctcgagttaa gaggtggcgg
       61 gtgcgggggc aacaagggcg ggtccagcag aggtaggggc ttcagatacg ggggcggcgc
      121 taacatgtcc atcgccatca ggattttcac ctaaccagga ggcaacttct tcaggagata
      181 acaggaggtg gtccattgga ggggaaaggg aatcggataa aacgttattt tcgggcagta
      241 atttccataa atcgctaaat gtttcttggg ataagggggg ttcaatacta agatcggagt
      301 gtggttcttc catcatatgg ctgccgcgcg gcaccaggcc gctgctgtga tgatgatgat
      361 gatggctgct gcccatggta tatctccttc ttaaagttaa acaaaattat ttctagaggg
      421 gaattgttat ccgctcacaa ttcccctata gtgagtcgta ttaatttcgc gggatcgaga
      481 tctcgatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg
      541 ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca
      601 tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg
      661 ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac
      721 tgggc
//
